Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. Experimental setup (see text for details). Figure Legend: From: High heterogeneity.

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Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. Experimental setup (see text for details). Figure Legend: From: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells J. Biomed. Opt. 2009;14(3): doi: /

Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. Fluorescence spectra emission: Red curve for a nanomolar concentration of DiA in DMSO (the narrow lines correspond to the Raman scattering of DMSO); green curve for DiA into the plasma membrane of LR73 cells (see text for labeling conditions). The fluorescence spectrum of DiA in DMSO is similar to the one in water, with an emission maximum ≈620nm in DMSO and ≈610nm in water. Inset: Typical confocal fluorescence picture of LR73-S labeled with DiA. (Color online only.) Figure Legend: From: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells J. Biomed. Opt. 2009;14(3): doi: /

Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. (a) and (b): Two typical experimental autocorrelation functions (ACFs) obtained for DiA-labeled LR73 cells (see text for labeling conditions). (c): Typical ACF recorded for a giant unilamellar vesicle (GUV) labeled with DiA (see text for labeling conditions). Black curves: Experimental autocorrelation curves. Blue curves: Total fit according to Eq.. Red curves: Fit according to a single class of molecules diffusing in two dimensions. (Color online only.) Figure Legend: From: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells J. Biomed. Opt. 2009;14(3): doi: /

Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. Diffusion-time distributions obtained through a cell-by-cell measurement, from two populations of 110 LR73-cells each, sensitive line (gray columns) and resistant line (red-striped columns) (see text for labeling conditions). These histograms have been built with a column bin size equal to the highest error (i.e., highest uncertainty) assessed through the autocorrelation curve fitting, here 0.5ms. Inset: The corresponding cumulative frequency profiles for LR73-S (black line) and LR73-R (red line). (Color online only.) Figure Legend: From: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells J. Biomed. Opt. 2009;14(3): doi: /

Date of download: 7/11/2016 Copyright © 2016 SPIE. All rights reserved. Diffusion-time distributions and cumulative frequency profiles of LR73 cells and effect of membrane agents. (a): Diffusion-time cumulative-frequency profiles for LR73-S untreated (red line) and treated with benzyl alcohol (10nM); (black line). Inset: The corresponding normalized diffusion-time distributions with the same color code (column bin size is 1.5ms). (b): Cumulative frequency profiles for LR73-R untreated (red line), treated with BA (10mM, 5min; black line), verapamil (5μM, 5min; blue line), and cyclosporin-A (5μM, 5min green line). Inset: The corresponding normalized diffusion-time distributions with the same color code. Diffusion-time cumulative frequency profiles for GUVs are also plotted in each graphic (black dottes curves). (Color online only.) Figure Legend: From: High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells J. Biomed. Opt. 2009;14(3): doi: /