CASE 3 ——Maize Genome-Wide and Organ-Specific Landscapes of Epigenetic Modifications and Their Relationships to mRNA and Small RNA Transcriptomes in Maize.

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CASE 3 ——Maize Genome-Wide and Organ-Specific Landscapes of Epigenetic Modifications and Their Relationships to mRNA and Small RNA Transcriptomes in Maize. The plant cell. 2009, 21(4): National Institute of Biological Sciences ， Yale University ， Beijing Genomics Institute at Shenzhen etc Research Objective Genome-Wide of Epigenetic Modifications in maize through high- throughput sequencing technologies research Material and methods Plants were grown under controlled environmental conditions(15 h light/258C, 9 h dark/208C) in a growth chamber The ChIPed DNA from three reactions was pooled to construct Solexa libraries essentially following the manufacturer’s standard protocol Results 1. It's system analysis how to comprehensive influence gene expression in four kinds of histone modification and DNA methylation. 2. We found an organ-specific distribution of canonical microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs), indicative of their tissue-specific biogenesis. 3. Found the two kinds of new siRNA