Date of download: 7/12/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Inhibition of the Growth of Papillary Thyroid Carcinoma.

Slides:



Advertisements
Similar presentations
Isosteviol derivatives induced apoptosis in Human lung cancer via targeting MEK/MAPK pathway: An in vitro and in vivo study Ahmed M Malki 1,,PhD Stephen.
Advertisements

Date of download: 5/27/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Molecular Targeting of Ultrasonographic Contrast.
Date of download: 5/30/2016 Copyright © 2016 American Medical Association. All rights reserved. From: The Common Inhalational Anesthetic Sevoflurane Induces.
Date of download: 6/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Biological Effects of Bexarotene in Cutaneous T-Cell.
Date of download: 6/22/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Hyaluronan and the Interaction Between CD44 and Epidermal.
Date of download: 6/22/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Reduction of Hyaluronan-CD44–Mediated Growth, Migration,
Date of download: 6/22/2016 Copyright © 2016 American Medical Association. All rights reserved. From: An In Vivo Evaluation of Docetaxel Delivered Intratumorally.
Date of download: 6/24/2016 Copyright © The American College of Cardiology. All rights reserved. From: Inhibition of ErbB2 causes mitochondrial dysfunction.
Date of download: 7/2/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Imatinib-Mediated Inactivation of Akt Regulates ABCG2.
Date of download: 7/9/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Follicular Variant of Papillary Thyroid Cancer: Encapsulated,
Date of download: 9/17/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Differential Responses of Human Papillary Thyroid.
Date of download: 9/17/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Cisplatin-Induced Growth Arrest of Head and Neck.
Copyright © 2009 American Medical Association. All rights reserved.
Copyright © 2009 American Medical Association. All rights reserved.
Copyright © 2007 American Medical Association. All rights reserved.
Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy  Flavia.
Src-Family Kinases Are Activated in Non-Small Cell Lung Cancer and Promote the Survival of Epidermal Growth Factor Receptor-Dependent Cell Lines  Jie.
From: Lectin from Agaricus bisporus Inhibited S Phase Cell Population and Akt Phosphorylation in Human RPE Cells Invest. Ophthalmol. Vis. Sci ;53(12):
From: Activation of the Rho/Rho Kinase Signaling Pathway Is Involved in Cell Death of Corneal Endothelium Invest. Ophthalmol. Vis. Sci ;57(15):
Copyright © 2012 American Medical Association. All rights reserved.
Copyright © 2006 American Medical Association. All rights reserved.
Copyright © 2008 American Medical Association. All rights reserved.
Copyright © 2008 American Medical Association. All rights reserved.
Copyright © 2009 American Medical Association. All rights reserved.
Copyright © 2003 American Medical Association. All rights reserved.
Copyright © 2010 American Medical Association. All rights reserved.
From: IGF-1 Regulates the Extracellular Level of Active MMP-2 and Promotes Müller Glial Cell Motility Invest. Ophthalmol. Vis. Sci ;56(11):
The Autophagy Inhibitor Chloroquine Overcomes the Innate Resistance of Wild-Type EGFR Non-Small-Cell Lung Cancer Cells to Erlotinib  Yiyu Zou, PhD, Yi-He.
Copyright © 2006 American Medical Association. All rights reserved.
Bovine lactoferricin B induces apoptosis of human gastric cancer cell line AGS by inhibition of autophagy at a late stage  W.-R. Pan, P.-W. Chen, Y.-L.
Dual Inhibition of PI3K/AKT and MEK/ERK Pathways Induces Synergistic Antitumor Effects in Diffuse Intrinsic Pontine Glioma Cells  Y. Linda Wu, Uday Bhanu.
Amanda M. Nelson, Kathryn L. Gilliland, Zhaoyuan Cong, Diane M
Vemurafenib Induces Senescence Features in Melanoma Cells
by Pascal Gelebart, Mona Anand, Hanan Armanious, Anthea C
Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor–κB and IκBα kinase in human multiple myeloma cells, leading to.
The TCL1 oncoprotein inhibits activation-induced cell death by impairing PKCθ and ERK pathways by Gilles Despouy, Marjorie Joiner, Emilie Le Toriellec,
G protein–coupled estrogen receptor 1 agonist G-1 induces cell cycle arrest in the mitotic phase, leading to apoptosis in endometriosis  Taisuke Mori,
Yongping Shao, Kaitlyn Le, Hanyin Cheng, Andrew E. Aplin 
MET Tyrosine Kinase Inhibitor Crizotinib (PF ) Shows Differential Antitumor Effects in Non-small Cell Lung Cancer According to MET Alterations 
Droxinostat, a Histone Deacetylase Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cell Lines via Activation of the Mitochondrial Pathway and.
Aurora kinase inhibitory VX-680 increases Bax/Bcl-2 ratio and induces apoptosis in Aurora-A-high acute myeloid leukemia by Xue-Fei Huang, Shao-Kai Luo,
Bertrand Poussier, MD, Alfredo C
RAF Inhibition Overcomes Resistance to TRAIL-Induced Apoptosis in Melanoma Cells  Anja Berger, Sandra-Annika Quast, Michael Plötz, Nicholas-Frederik Kuhn,
Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway  Tatsuo Nakahara, M.D., Akira Iwase, M.D., Ph.D.,
Jinu Kim, Kelly E. Long, Kang Tang, Babu J. Padanilam 
TNF enhances dose‐dependent cell death following doxorubicin‐induced DNA damage with minimal affect on dose‐dependent cell‐cycle arrest. TNF enhances dose‐dependent.
G protein–coupled estrogen receptor 1 agonist G-1 induces cell cycle arrest in the mitotic phase, leading to apoptosis in endometriosis  Taisuke Mori,
Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy  Flavia.
The Autophagy Inhibitor Chloroquine Overcomes the Innate Resistance of Wild-Type EGFR Non-Small-Cell Lung Cancer Cells to Erlotinib  Yiyu Zou, PhD, Yi-He.
Volume 137, Issue 3, Pages (September 2009)
AT-101, a Pan-Bcl-2 Inhibitor, Leads to Radiosensitization of Non-small Cell Lung Cancer  Luigi Moretti, MD, Bo Li, MD, Kwang Woon Kim, PhD, Heidi Chen,
Terameprocol (Tetra-O-Methyl Nordihydroguaiaretic Acid), an Inhibitor of Sp1-Mediated Survivin Transcription, Induces Radiosensitization in Non-small.
BV6, an IAP Antagonist, Activates Apoptosis and Enhances Radiosensitization of Non- small Cell Lung Carcinoma In Vitro  Wenyan Li, MD, PhD, Bo Li, MD,
Inhibition of β-Catenin Enhances the Anticancer Effect of Irreversible EGFR-TKI in EGFR-Mutated Non–small-cell Lung Cancer with a T790M Mutation  Yosuke.
Dasatinib, a small molecule inhibitor of the Src kinase, reduces the growth and activates apoptosis in pre-neoplastic Barrett's esophagus cell lines:
Volume 82, Issue 7, Pages (October 2012)
Activation of Akt as a Mechanism for Tumor Immune Evasion
Volume 16, Issue 24, Pages (December 2006)
Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis  Hsin-Su Yu, Gwo-Shing Chen 
Volume 41, Issue 6, Pages (December 2004)
MEK inhibitor, U0126, attenuates cisplatin-induced renal injury by decreasing inflammation and apoptosis  Sang-Kyung Jo, Won Yong Cho, Su Ah Sung, Hyoung.
FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.
BCR–ABL kinase activity rewires GM-CSFR signaling and confers oncogene addiction in TF1 cells. BCR–ABL kinase activity rewires GM-CSFR signaling and confers.
Roland Houben, Sonja Ortmann, David Schrama, Marco J
Effects of knockdown of ANRIL on NSCLC cell cycle and apoptosis in vitro. Effects of knockdown of ANRIL on NSCLC cell cycle and apoptosis in vitro. A,
Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.
LY induces cytotoxicity in hMECs via inhibition of Akt/p70S6K kinase activities. LY induces cytotoxicity in hMECs via inhibition of Akt/p70S6K.
N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.
RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis.
Quercetin induces autophagy, blocks cell cycle and activation of ERK and JNK signaling pathways participates in the G1 arrest in P39 cells. Quercetin induces.
Presentation transcript:

Date of download: 7/12/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Inhibition of the Growth of Papillary Thyroid Carcinoma Cells by CI-1040 Arch Otolaryngol Head Neck Surg. 2009;135(4): doi: /archoto CI-1040 potently inhibits papillary thyroid carcinoma (PTC) cell growth in vitro. The PTC cells were treated with CI-1040 for up to 4 days. CI-1040 was added to the cells on day 0. Each day, the cells were counted according to absorption at 570 nm (Abs 570) after staining with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT using a microplate reader. Cells treated with dimethyl sulfoxide (DMSO) only were used as positive controls. The CI-1040 concentrations used were 0.5μM (10 × concentration needed to inhibit 50% cell growth [GI 50 ]), 0.05μM (GI 50 ), and 0.005μM (0.1 × GI 50 ) for PTC cells with a BRAF mutation (A) and 10μM (10 × GI 50 ), 1μM (GI 50 ), and 0.1μM (0.1 × GI 50 ) for PTC cells with the RET/PTC1 rearrangement (B). Figure Legend:

Date of download: 7/12/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Inhibition of the Growth of Papillary Thyroid Carcinoma Cells by CI-1040 Arch Otolaryngol Head Neck Surg. 2009;135(4): doi: /archoto CI-1040 induces G1 arrest in RET/PTC1–rearranged and BRAF-mutated papillary thyroid carcinoma (PTC) cells. The PTC cells (RET/PTC1 rearranged or BRAF mutated) were treated with 1μM CI-1040 for 24 hours. Cells treated with dimethyl sulfoxide (DMSO) were used as a control. A, Flow cytometry data. M1 is the G1/G0 phase; M2, the G2/M phase; M3, the S phase; and M4, the apoptotic phase. B, The percentage of cells in the G1/G0, G2/M, S, and apoptotic phases after flow cytometry from 3 independent experiments. Error bars represent SD. Figure Legend:

Date of download: 7/12/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Inhibition of the Growth of Papillary Thyroid Carcinoma Cells by CI-1040 Arch Otolaryngol Head Neck Surg. 2009;135(4): doi: /archoto The extent and duration of dephosphorylation of extracellular signal–related kinase 1/2 (ERK1/2) after CI-1040 treatment depend on papillary thyroid carcinoma (PTC) genotype. The expression of phosphorylated ERK1/2 (p-ERK1/2) was detected using Western blot analysis. Total ERK1/2 and actin were used as loading controls, and cells treated with dimethyl sulfoxide (DMSO) only were used as positive controls for normal expression of p-ERK1/2. A, Cells with a BRAF mutation were treated with CI-1040 at 0.001μM, 0.01μM, 0.05μM, 0.1μM, 1μM, or 5μM for 1 hour (top) or with 1μM CI-1040 for 1, 6, 24, 48, 72, or 96 hours (bottom). B, Cells with the RET/PTC1 rearrangement were treated with 0.001μM, 0.01μM, 0.1μM, 1μM, or 5μM CI-1040 for 1 hour (top) or with 1μM CI for 1, 2, 4, 6, 8, 24, 48, or 72 hours (bottom). Figure Legend:

Date of download: 7/12/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Inhibition of the Growth of Papillary Thyroid Carcinoma Cells by CI-1040 Arch Otolaryngol Head Neck Surg. 2009;135(4): doi: /archoto Evidence of apoptosis in papillary thyroid carcinoma (PTC) cells with a BRAF mutation after CI-1040 treatment. A, Detection of cleaved caspases and cleaved poly (ADP-ribose) polymerase (PARP) in cells with a BRAF mutation after CI-1040 treatment. Cells with a BRAF mutation were treated with 1μM CI-1040 for 1 to 4 days. Expression of cleaved caspase-9, caspase-3, and cleaved PARP was detected by means of Western blot analysis. Cells treated with dimethyl sulfoxide (DMSO) only were used as controls. Actin was used as a loading control. B, The PTC cells with a BRAF mutation or the RET/PTC1 rearrangement were treated with 2μM CI-1040 for 3 or 4 days. Cells were harvested, and apoptotic cells were labeled using a fluorometric TUNEL (terminal deoxynucleotide transferase–mediated dUTP nick-end labeling) system. After analyzing cells using flow cytometry, the green cells that appeared in the R2 region (apoptotic cells) were counted, and the percentage of TUNEL-positive cells are shown. Cells treated with DMSO only (Mock) were used as a negative control, and cells treated with 40μM cisplatin for 48 hours were used as a positive control. Figure Legend: