Outline History(TEM) Background Components Specimen Preparation Imaging method Contrast formation Modifications STEM References.

Slides:

Advertisements

Similar presentations

Microscopy Do you want a footer?.

Advertisements

A Question from Last Year Final Exam

3.1 AS Unit F211: Cells, Exchange and Transport The cell is the basic unit of all living things. How to use a light microscope Why electron microscopes.

SEM & TEM in Polymer Characterization

Lecture 11. Microscopy. Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single or.

Do it with electrons ! II.

Scanning Electron Microscope (SEM)

IFFAT FATIMA UOG. ELECTRON MICROSCOPE Contents History LM Vs EM Electron microscope Principle Types of EM Application & importance.

Lecture 5.1 Scanning Electron Microscopy (SEM)

Electron Microscope. Light Resolution  The resolution of a microscope is limited by the diffraction of light. Single diffractionSingle diffraction 

Activities during UK-Japan Young Scientist Workshop Dr Riz Khan Room 31DJ02, x6062, Advanced Technology Institute University.

FYSZ460 Electron Beam Lithography Electron Beam Lithography FYSZ460 Advanced Laboratory Exercise Mikko Palosaari

Types of Microscopes Microscopes Rule!!!!!!!!!!. There are four basic kinds of microscopes: Optical (or light) Electron Scanning Probe Ion.

Microscopes are used to increase the magnification and resolving power of the unaided eye MICROSCOPES.

SEM microscope By: Doug, Holly & Oleg. Scanning Electron Microscope vs. Optical Microscope Advantages Continuously variable magnification High resolution.

Do it with electrons !. Microscopy Structure determines properties We have discussed crystal structure (x-ray diffraction) But consider now different.

Electron Microscope. Light vs EM Which is which? What are the main differences?

Cytoplasmic Organelles Plastids Plant organelle that may take many forms. Examples include chloroplast, leukoplasts (which store food), & chromoplasts.

Honors Microbiology: Chapter 3 Microscopy and Staining

Naomi Kinjal Asaad Binoy

Introduction to the Cell

Cycling of Matter in Living Systems 1.3 Developments in Imaging Technology and Staining Techniques.

Scanning Electron Microscope (SEM)

Other modes associated with SEM: EBIC

Scanning Electron Microscope (SEM)

Advanced Biology Visualizing Cells. The Human Eye  Resolution – The minimum distance two points can be apart and still be distinguished as two separate.

MICROSCOPES Some Important Vocabulary MAGNIFICATION- increase of an object’s apparent size RESOLUTION- the power to show details clearly * Microscopes.

Looking at Cells Section 3.1.

TEM charcaterization Basic modes – Bright field microscopy – Dark field Microscopy –STEM – EDAX – EELS.

Tools in Science Miss Colabelli. Tools & Techniques Tools are objects to improve the performance of a task. Microscopes are tools that extend human vision.

Reminders for this week Homework #4 Due Wednesday (5/20) Lithography Lab Due Thursday (5/21) Quiz #3 on Thursday (5/21) – In Classroom –Covers Lithography,

Unit 4: Cells Microscopes and the Discovery of Cells.

Microscopia de Iones y Nano-Tecnología Eduardo H. Montoya Rossi.

first compound microscope – Zacharias Jansen in 1590

SEM Scanning Electron Microscope

Cell Theory and the Microscope SBI3C0 Ms. Manning.

Chapter 1 Section 4 Tools and Techniques (specifically microscopes) p

Tools of a Biologist MICROSCOPY Two factors play an important role in microscopy: 1. Magnification compares real size of a specimen with the one viewed.

Questions/Problems on SEM microcharacterization Explain why Field Emission Gun (FEG) SEM is preferred in SEM? How is a contrast generated in an SEM? What.

A.E. GunnæsMENA3100 V08 Electron Diffraction (ED) in the transmissions electron microscope.

B-1.2: Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation.

Electron Microscope. How do they work Instead of using light they fire a beam of electrons (which have a wavelength less than 1nm compared to light which.

SARDAR PATEL INSTITUTE OF TECHNOLOGY E.NO : Guide By:- V.N.Thakkar.

Types of Electron Microscopes Electron microscopes use a beam of electrons rather than visible light to illuminate the sample. They focus the electron.

Scanning Transmission Electron Microscope

Do it with electrons !. Microscopy Structure determines properties We have discussed crystal structure (x-ray diffraction) But consider now different.

Microscopy Group 2 Cabatit, Mendoza, Ramos, Rodriguez, Tan.

THE ELECTRON MICROSCOPE. Introduction.

Electron Microscopes. We will be discussing The history of the electron microscope Two major difference between the electron microscope and light microscope.

Chapter 2: Viewing the Microbial World

Transmission Electron Microscope

1.4 Tools & Techniques Microscopes– tools that extend human vision by making enlarged images of objects Magnification – power to increase the size of an.

Presentation on SEM (Scanning of Electron Microscope) Represented by:-Ravi Kumar Roll:- (BT/ME/1601/006)

TRANSMISSION ELECTRON MICROSCOPy (TEM).

Laboratory equipment Lecture (3).

Introduction to Scanning Electron Microscope by Sameer S

3.3 Other types of microscopy

Investigation of the microstructure in the Yttrium-Tantalate-Zirconia system Thursday, August 27, 2015.

TEM (Transition Electron Microscope)

Topic 1: Introduction to Histology

By Dominique Baker & Moyna John

Do it with electrons !.

A C B D 1. Read the information about different sorts of microscope.

Electronics and Instrumentation Vidnyan Mahavidyalaya, Sangola.

Types of Microscopy Type Probe Technique Best Resolution Penetration

Scanning Electron Microscopy (SEM)

The Microscope Appendix C pp.4-5

Presentation transcript:

Outline History(TEM) Background Components Specimen Preparation Imaging method Contrast formation Modifications STEM References

Scanning Transmission Electron Microscope

Specimen Preparation Prepare a specimen with at least part of its thickness at about 100 nm, depending on the atomic weight of specimen materials. Pre-Thinning:  1 st specimen less than 1mm thick is prepared  Reducing the thickness to about 0.1mm before final thinning to 100 nm thickness Final Thinning: involve  Ion Milling  Electrolytic Thinning  Ultramicrotomy

Ion Milling Uses a beam of energetic ions to bombard specimen surfaces to reduce the thickness by knocking atoms out of a specimen General procedure a)Dimple grinding b)ion milling  ion beam of 1–10 keV bombarded  specimen is placed in the center at an angle of 5-30◦

Electrolytic Thinning Reducing specimen thickness to 100nm General procedure  A specimen placed in an electrochemical cell as anode  A suitable reduce specimen thickness  Common technique is jet polishing  Electrolytic thinning completed in 3–15 minutes.

Ultramicrotomy Reducing specimen thickness to 100nm General procedure  A specimen is mounted in a holder against the cutting tool  The specimen should be trimmed to have a tip held against the knife  The holder gradually moves toward the knife while it repeatedly moves up and down

STEM The basic principle of image formation fundamentally different from static beam TEM small spot size is formed on the sample surface with the condenser lenses This probe is scanned on the sample surface the signal is detected by an electron detector, amplified and synchronously displayed on CRT with the scan coils

Cont… DETECTOR 1.Small disk on the column axis which detects the transmitted beam (BF STEM image) or diffracted beam (DF STEM image) 2. Annular detector (a plate with a hole) which detects all the diffracted beams except the transmitted one (ADF STEM) Resolution  limited by the spot size  have poorer resolution but better contrast

MATERIALS CHARACTERIZATION Introduction to Microscopic and Spectroscopic Methods by Yang Leng -ISBN