#AIDS2016 HIV Persists in Colon and Blood CCR6+ T-Cells During Viral Suppressive ART Jean-Pierre Routy on behalf of : A. Gosselin, T.

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Presentation transcript:

#AIDS2016 HIV Persists in Colon and Blood CCR6+ T-Cells During Viral Suppressive ART Jean-Pierre Routy on behalf of : A. Gosselin, T Wiche Salinas, D. Planas, V. Sue Wacleche, Y. Zhang, R. Fromentin, N. Chomont, E. Cohen, B. Shacklett, V. Mehraj, M. Ghali and Petronela Ancuta Université de Montréal, CRCHUM, Montréal, Québec, Canada McGill University Health Centre, Montréal, Québec, Canada 21st International AIDS Conference Durban, 21 July 2016

#AIDS2016 Preferred CD4 T cells for HIV persistence Kulpa J of virus eradication 2015 Fromentin, Chomont AIDS 2016 Contribution for HIV reservoir: Tissue distribution Frequency of the CD4 subsets Replication competent Activation: DR Exhaustion: PD-1, TIGIT. Lag3 Th phenotypes Th1/Th17 & Th17: Mucosa defence Surface marker: CCR6+ Gosselin/Monteiro, J Immunol, 2010 Montiriro J immunol 2011 Cell differentiation

#AIDS2016 CCR6 + CD4 + T-Cells are Enriched in the Colon of HIV-Infected Individuals During ART A Lineage (CD8, CD19, CD66b) CD326CD3 Relative cell counts CD45RA CD4 CD3 CCR6 All viable cells Lineage- cells CD3+ T-cells CD4+CD45RA- PBMC Colon Gosselin/Wiche Salinas et al. submitted B: Patients HIV+ on ART biopsy EC, elite controller p=0.03 p= Rapid CCR6 T cell depletion in blood Not restored by ART Persists via Bcl2 expression CCR6+

#AIDS2016 CCR6+CD4+ T-Cells are Preferentially Infected in the Colon and Blood of HIV-Infected Individuals During ART Gosselin/Wiche Salinas et al. submitted B, HIV+ on ART biopsy EC, elite controller Blood

#AIDS2016 Blood CCR6+CD4+ T-Cells Harbor Replication-Competent HIV Day 0Day 3Day 6Day 9 MACS/FACS cell isolation TCR triggering ± ATRA (2x10e6 cells/well) Harvest media Add IL-2 ± ATRA IL-17A ELISA HIV-p24 ELISA Harvest media Add IL-2 ± ATRA HIV-p24 ELISA Harvest media and cells HIV-p34 ELISA HIV-p24 ICS Gosselin/Wiche Salinas et al. submitted

#AIDS2016 Acknowledgements CHUM-Research Centre Petronela Ancuta Annie Gosselin Tomas Wiche Salinas Delphine Planas, MSc Vanessa Sue Wacleche, MSc Amélie Cattin, MSc Yuwei Zhang, PhD Huicheng Chen, PhD Sylvia Pouvreau Nicolas chomont Rémi Fromentin, PhD Mohamed El-Far, PhD CanCURE : Robert Reinhard CHUM-Research Centre Flow Cytometry Core Facility Dominique Gauchat, PhD McGill University Health Centre Vikram Mehraj, Peter Ghali, Josée Girouard FRQS AIDS Network, Mario Legault, Cecile Tremblay, Mark Wainberg, Michel Roger IRCM, CanCURE: Eric Cohen, Sebastien Sebbagh Volunteers for their gift of leukapheresis and gut biopsies