Who is the Solitary Long Terminal Repeats of ERV-9 Endogenous Retrovirus? 석사 1 년 김 우 연.

Slides:

Advertisements

Similar presentations

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

Advertisements

Describe the structure of a nucleosome, the basic unit of DNA packaging in eukaryotic cells.

Copyright, ©, 2002, John Wiley & Sons, Inc.,Karp/CELL & MOLECULAR BIOLOGY 3E The Stability of the Genome Duplication, Deletion, Transposition.

We have shown that: To see what this means in the long run let α=.001 and graph p:

Promoters Map ends of mRNA on DNA Mapping sites on DNA for protein binding General Properties of promoters Bacterial Promoters Promoters for eukaryotic.

Studying Gene Expression and Function

Molecular Biology Topics for Second Part of Molecular Biology Course: Structural Organization of Eukaryotic Chromosomes Spring 2009 BI 425 UOG.

Chapter 19: Eukaryotic Genomes Most gene expression regulated through transcription/chromatin structure Most gene expression regulated through transcription/chromatin.

Plant Molecular Systematics Michael G. Simpson

Cloning and rDNA (II) Dr. Abdulaziz Almalik

RNA and Protein Synthesis

Alternative splicing and promoter of the GSDML gene implicated in the HERV-H LTR element Kung-Ahn, Jae-Won Huh, Dae-Soo Kim, Hong-Seok Ha, Yun-ji Kim,

Regulation of Gene Expression Chapter 18. Warm Up Explain the difference between a missense and a nonsense mutation. What is a silent mutation? QUIZ TOMORROW:

Ch. 21 Genomes and their Evolution. New approaches have accelerated the pace of genome sequencing The human genome project began in 1990, using a three-stage.

Sergey G. Kurdyukov a, Yuri B. Lebedev a, Irena I. Artamonova a, Tatyana N. Gorodentseva a, a Anastasia V. Batrak a, Ilgar Z. Mamedov a, Tatyana L. Azhikina.

1 Genome Evolution Chapter Introduction Genomes contain the raw material for evolution; Comparing whole genomes enhances – Our ability to understand.

Centra Dogma Primer. Structure of DNA and RNA Nucleic acids made of nucleotides G, A, T/U, C Ribose vs. deoxyribose Template-dependent synthesis Double.

Regulating Eukaryotic Gene Expression. Why change gene expression? Different cells need different components Responding to the environment Replacement.

HA Hong-seok, HUH Jae-Won, KIM Dae-Soo 1, JOO Myung-Jin 2 and KIM Heui-Soo* Division of Biological Sciences, College of Natural Sciences, Pusan National.

HERVs (Human endogenous retroviruses) and LTR (long terminal repeat) - like elements are dispersed over 8% of the whole human genome. There are at least.

MCDB 4650 Developmental Control of Gene Expression.

REFERENCES 1. J. Boeke, J. Stoye, Retrotransposons, endogenous retroviruses, and the evolution of retroelements, In Retroviruses (1997) 343– J.

Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein.

Figure S1. Alignment of sequences from the 5′-end to the Sm binding site of reported genomic sequences (9-15) for HSUR 1. MicroRNA binding sites are.

Gene Transfer. Comparison of different viral vectors Viral vectortitersmanupilation of immunogenicityinfecting of tropism non-dividing cells Adenovirus10.

ABSTRACT Isolation and phylogeny of endogenous retroviral elements belonging to the HERV-K LTR in cDNA library of human fetal brain and X q 21.3 region.

MPL The DNA Sequence of chimpanzee chromosome 22 and comparative analysis with its human ortholog, chromosome 21 Bioinformatics Dae-Soo Kim.

Evolution at the Molecular Level. Outline Evolution of genomes Evolution of genomes Review of various types and effects of mutations Review of various.

CFE Higher Biology DNA and the Genome Transcription.

Genome Editing by Matthew Porteus Department of Pediatrics,

Genetic Code and Interrupted Gene Chapter 4. Genetic Code and Interrupted Gene Aala A. Abulfaraj.

Molecular Tools for Studying Genes and Gene Activity

Transcription Basic Methods

Schematic representation of mechanisms of activation of a host gene by insertion of a provirus and the general structure of leukemia and leukosis and acute.

Yu-Na Noh1, Jae-Won Huh1, Dae-Soo Kim2, Kung Ahn1, and Heui-Soo Kim1,2

Molecular Evolution of PPHLN1 Gene in Relation to HERV-M Element

Emily Eder HC70AL - Spring 2005

Detection of genome regulation sequences

Control of Gene Expression

Motifs BCH364C/394P - Systems Biology / Bioinformatics

Transposable elements and Cancer

Gene-related Sequence

9.11 Viruses That Use Reverse Transcriptase

Different expression pattern between human and primate by integration of LTR33 element in MOBP gene Yu-Na Noh1, Jae-Won Huh2, Dae-Soo Kim3, Hong-Seok Ha1,

Evolution of eukaryote genomes

Pusan National University

GENOME INFORMATION LABORATORY

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

Gene Density and Noncoding DNA

Isolation and Chracterization of the Human Syncytin Gene Promoter

Volume 146, Issue 6, Pages (September 2011)

Volume 3, Issue 4, Pages (April 2013)

Fig. 6. HERV-K activation by TDP-43 and identification of binding sites on LTR. (A and B) Stem cell–derived neurons were transfected with either pcDNA.

GENOME INFORMATION LABORATORY

Hannah K. Long, Sara L. Prescott, Joanna Wysocka Cell

Comparative Transcription Promoter Activity

The gene: structure, function and location

Hong-Seok Ha1, Jae-Won Huh1, Dae-Soo Kim2, and Heui-Soo Kim1 2

Unit Genomic sequencing

Possible role of c-Jun in transcription of the mouse renin gene

Defining the Regulatory Elements in the Proximal Promoter of ΔNp63 in Keratinocytes: Potential Roles for Sp1/Sp3, NF-Y, and p63 Rose-Anne Romano, Barbara.

Motifs BCH339N Systems Biology / Bioinformatics – Spring 2016

Promoting in Tandem: The Promoter for Telomere Transposon HeT-A and Implications for the Evolution of Retroviral LTRs O.N Danilevskaya, I.R Arkhipova,

The endogenous IGRP gene is selectively expressed in βTC-3 cells and not in Y1 cells. βTC-3 RNA (50 μg) and Y1 RNA (50 μg) were annealed to labeled oligonucleotide.

Relationship between Genotype and Phenotype

Transcriptional Regulation by p53 through Intrinsic DNA/Chromatin Binding and Site- Directed Cofactor Recruitment Joaquin M Espinosa, Beverly M Emerson

Gene regulatory regions of the insect/crustacean egr-B homologs.

Genome Information Lab

Engineering Globin Gene Expression

Presentation transcript:

Who is the Solitary Long Terminal Repeats of ERV-9 Endogenous Retrovirus? 석사 1 년 김 우 연

Contents Identification of ERV9 LTR Identification of R within ERV9 LTR Mutational Analysis of ERV9 Promoter Region Mobilization of ERV9 during Primate Evolution ERV9 LTR in β-globin gene and Axin gene Evolution of ERV9 Future study

1. Identification of ERV9 in undifferentiated embryonal carcinoma cells

Assay of ERV9 LTR promoter activity in different human cell lines

2. Identification of R within ERV9 LTR Identification of R within HERV9 LTR ( LTR12 )

Identification of transcription start sites - Transfection in NT2/D1 cells - Total RNA isolation - Primer extension analysis Using a primer complementary to the CAT coding region

CAT expression assay CAT gene Chloramphenicol Acetyltransferase counting the amount of [14C] Chloramphenicol converted to the mono-acetylated forms Efficient promoter activity region : -70/+6, -70/-7

DNase I footprint analysis : +7 ~ -10 : -33 ~ -54 Sp1 transcription factor binds to -54 ~ -33

Sp1 transcription factor binds to -54 ~ -33

3. Mutational Analysis of ERV9 Promoter Region

The potential of different transcription factors

4. Mobilization of ERV9 during Primate Evolution

zinc finger protein 80 (ZNF80) gene

ZNF80 in the primate species

Alignment of LTRs regions

Phylogenetic tree of radiation, insertion time Insertion time of the ERV9 element

5. ERV9 LTR in β-globin gene and Axin gene ERV9 LTR is conserved during primate evolution and possess enhancer activities

β-globin gene region A. B. nuclease hypersensitive site 5,4,3,2,1 HBE1, HBG2,1 HBBP1, HBD, HBB B. Human, Chimpanzee, Gorilla, Orangutan – LTR12 Gibbon, Monkey – no LTR12

Axin gene - HESAS Result of HESAS

Axin gene – axin LTR

By FACS

Enhancer and promoter activities

6. Evolution of HERV9

Identified ERV9 Elements Synapomorphic combination of character states i.e. Diagnostic nucleotide substritutions

Ages of ERV9 Subfamilies

Phylogenetic relationships

Divergence

Future study ERV9 LTR ( LTR11, LTR12, LTR17, pTR5 ) 분석 U5 영역의 repeat sequence 분석 Human genome 전체에서 ERV9 LTR 삽입 상태 분석 Gene 과 연결된 ERV9 LTR 분석 Gene 과 연결된 상태에 따라 분석 ERV9 LTR의 Expression 분석