Date of download: 9/17/2016 Copyright © 2016 American Society of Anesthesiologists. All rights reserved. Fig. 3. Activities of superoxide dismutase, catalase,

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Date of download: 9/17/2016 Copyright © 2016 American Society of Anesthesiologists. All rights reserved. Fig. 3. Activities of superoxide dismutase, catalase, and content of malondialdehyde in the serum. (A ) A higher activity of SOD was found in the RIPC/no DMTU group compared with that in the other three groups at 20 min after the RIPC (P = 0.001), 20 min of ischemia (P = 0.001), and 20 min (P = 0.001) and 6 h after reperfusion (P = 0.001). SOD activities in the no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU groups decreased at 20 min of ischemia (P = 0.001) and 20 min after reperfusion (P = 0.001) compared with preischemic state. (B ) A higher activity of catalase was found in the RIPC/no DMTU group compared with that in the other three groups at 20 min after the RIPC (P = 0.001), 20 min of ischemia (P = 0.001), and 20 min (P = 0.001) and 6 h after reperfusion(P = 0.001). Catalase activities in the no RIPC/no DMTU (P = 0.012), no RIPC/DMTU (P = 0.011), and RIPC + DMTU (P = 0.012) groups decreased at 20 min of ischemia compared with preischemic state. (C ) Malondialdehyde content in the serum in the RIPC/no DMTU group was increased at 20 min after RIPC (P = vs. preischemic level). Malondialdehyde content in the serum was significantly increased in the no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU groups at 20 min and 6 h after reperfusion (P = vs. preischemic level). The serum malondialdehyde content in the RIPC/no DMTU group was significantly lower than that in the other three groups at 6 h after reperfusion (P = 0.013, 0.011, and vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU, respectively). Data are presented as mean ± SD. *P < versus No RIPC/No DMTU, # P < 0.05 versus preischemic state. No RIPC/no DMTU = animal that received sham pretreatment before 20 min spinal cord ischemia; RIPC/no DMTU = animal that received two cycles of occlusion/reperfusion of bilateral femoral arteries for 10-/10-min interval 30 min before spinal cord ischemia; No RIPC/DMTU = animal that received sham pretreatment and dimethylthiourea administration (1 h before sham pretreatment) before 20 min spinal cord ischemia; RIPC + DMTU = animal that received RIPC and dimethylthiourea administration (1 h before pretreatment) before 20 min spinal cord ischemia. CAT = catalase; DMTU = dimethylthiourea; MDA = malondialdehyde; RIPC = remote ischemic preconditioning; SOD = superoxide dismutase; Pre = Pre RIPC; RIPC 20 min = 20 min after RIPC; I 20 min = 20 min of ischemia; R 20 min = 20 min after reperfusion; R 6 h = 6 h after reperfusion. Figure Legend: From: Limb Remote Ischemic Preconditioning Protects the Spinal Cord from Ischemia–Reperfusion Injury:A Newly Identified Nonneuronal but Reactive Oxygen Species–dependent Pathway Anesthesiology. 2010;112(4): doi: /ALN.0b013e3181d0486d From: Limb Remote Ischemic Preconditioning Protects the Spinal Cord from Ischemia–Reperfusion Injury:A Newly Identified Nonneuronal but Reactive Oxygen Species–dependent Pathway Anesthesiology. 2010;112(4): doi: /ALN.0b013e3181d0486d