Date of download: 9/17/2016 Copyright © 2016 American Society of Anesthesiologists. All rights reserved. Fig. 4. Activities of superoxide dismutase (A.

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Presented by R. Sruthi PhD Scholar Department of Pharmacy Under Supervision of Dr. Rajeev Taliyan Assistant Professor Department of Pharmacy.

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Date of download: 9/17/2016 Copyright © 2016 American Society of Anesthesiologists. All rights reserved. Fig. 4. Activities of superoxide dismutase (A ), catalase (B ), and content of malondialdehyde (C ) in the spinal cord. (A ) A higher activity of superoxide dismutase (SOD) was found in the spinal cord in the RIPC/no DMTU group compared with that in the other three groups at 20 min after RIPC (P = 0.034, 0.026, and vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU; respectively) and 6 h after spinal cord ischemia–reperfusion (P = vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU). SOD activities in the spinal cord in the RIPC/no DMTU group were decreased at 24 h after reperfusion (P = vs. preischemic level). (B ) A higher activity of catalase was found in the spinal cord in the RIPC/no DMTU group compared with that in the other three groups at 20 min after RIPC (P = 0.019, 0.021, and vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU; respectively) and 6 h after spinal cord ischemia–reperfusion (P = vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU). Catalase activities in the spinal cord in the RIPC/no DMTU group were decreased at 24 h after reperfusion (P = vs. preischemic level). (C ) Malondialdehyde content in the spinal cord was significantly increased at 6 and 24 h after reperfusion in all the four groups (P = vs. preischemic level). The spinal cord malondialdehyde content in the RIPC/no DMTU group was significantly lower than that in the other three groups at 6 h after reperfusion (P = vs. no RIPC/no DMTU, no RIPC/DMTU, and RIPC + DMTU). *P < 0.01 versus no RIPC/no DMTU, # P < 0.01 versus preischemic state. Data are presented as mean ± SD. No RIPC/No DMTU = animal that received sham pretreatment before 20 min spinal cord ischemia; RIPC/No DMTU = animal that received two cycles of occlusion/reperfusion of bilateral femoral arteries for 10-/10-min interval 30 min before spinal cord ischemia; No RIPC/DMTU = animal that received sham pretreatment and dimethylthiourea administration (1 h before sham pretreatment) before 20 min spinal cord ischemia; RIPC+DMTU = animal that received RIPC and dimethylthiourea administration (1 h before pretreatment) before 20 min spinal cord ischemia. CAT = catalase; DMTU = dimethylthiourea; MDA = malondialdehyde; RIPC = remote ischemic preconditioning; SOD = superoxide dismutase; Pre = Pre RIPC; RIPC 20 min = 20 min after RIPC; R 6 h = 6 h after reperfusion; R 24 h = 24 h after reperfusion. Figure Legend: From: Limb Remote Ischemic Preconditioning Protects the Spinal Cord from Ischemia–Reperfusion Injury:A Newly Identified Nonneuronal but Reactive Oxygen Species–dependent Pathway Anesthesiology. 2010;112(4): doi: /ALN.0b013e3181d0486d From: Limb Remote Ischemic Preconditioning Protects the Spinal Cord from Ischemia–Reperfusion Injury:A Newly Identified Nonneuronal but Reactive Oxygen Species–dependent Pathway Anesthesiology. 2010;112(4): doi: /ALN.0b013e3181d0486d