Enzymes General properties Enzyme definition Factors affecting enzyme activity. Enzyme Inhibition. Application of enzyme inhibition. Isoenzymes.

General notes Important in all body reactions. Specific biological catalysts. Accelerate the reactions.

Definition Enzyme : It is a protein catalyst synthesized in all living cells that regulate a biochemical reaction without being changed.

Enzyme Enzyme DEFINITION: Are proteins catalysts for biochemical reaction. Are proteins catalysts for biochemical reaction. General properties of enzymes 1.enzyme catalyze chemical reaction 2.they do not change the equilibrium point of the reaction that they catalyze 3..since all known enzyme are proteins any agency or electricity that can damage native protein structure also causes loss of catalytic activity, thus heating of enzyme, treatment with strong acid or bases or exposure to denaturationg agent destorage their catalytic activity.

4.enzymes have molecular weight ranging about to over one million, some enzymes consist only of one or more polypeptide chain,but other contain in addition some other chemical component that is required for activity called a cofactor. The cofactor may be either metal such as Mg,Mn, Zn, or Fe, or it may be complex organic molecular,usually called a coenzyme. Some enzymes required both a metal ion and a coenzyme

Nomenclature of enzyme 1.old system ase lipid lapase 2.mechanism and type of reaction; decarboxylase removal of co2 dehydogenasetransferas 3.international unit of biochem I.U.B. code no; E.C. class…subclass…sub.sub.class…specific enzyme

Classification of enzymes Class no;I Oxidoredoctase Class no;II Transferase Class no;III Hydrolases Class no;IV Lyases Class no; V Isomerases cis and Trans Class no ;VI Ligases