Bacterial Cultures *Bacteria grow best in warm, moist, dark areas that contain a lot of food. -When we culture bacteria, we provide them with this environment.

Slides:

Advertisements

Similar presentations

Kirby-Bauer disk diffusion Testing skill based learning

Advertisements

Growing microbes About ‘grow’ and ‘reproduce’ Growing microbes practical B Growing microbes.

Bacterial culture in laboratory

Lab procedures when handling micro-organisms Distribution of micro-organisms in nature.

Bacterial lawn Learning objectives

Year 5 Practical Microbiology Homework

PHT 381 Lab # 9. MIC: It is the lowest concentration of the antimicrobial agent that inhibits the growth of the test organism but not necessarily kills.

Microbial Growth.

STREAKING AN AGAR PLATE FOR ISOLATIONOF BACTERIAL COLONIES BACTERIAL COLONIES.

PHT 381 Lab # 9. MIC : - It is the lowest concentration of the antimicrobial agent that inhibits the growth of the test organism but not necessarily kills.

Serial dilution and colony counting ● Serial dilution and colony counting – Also know as “viable cell counts” – Concentrated samples are diluted by serial.

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Microbiology Safety w Microbes are present everywhere. w Harmful microbes should not be allowed to grow and develop w PRECAUTIONS w All surfaces should.

Key Area 6 : Growth in Micro-organisms

Determination of MIC by Agar Diffusion Method. Minimum Inhibitory Concentration (MIC)  Definition: is the lowest concentration of an antimicrobial agent.

L8 – Keeping things sterile

Antibiotic susceptibility testing Modified Kirby-Bauer method

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Introduction to Lab Ex. 19: Enumeration of Bacteria

G ROWING BACTERIA. The action of antibiotics and disinfectants can be investigated using cultures of microorganisms (populations of microorganisms that.

Evaluation of antimicrobial agents and Neisseria species Lab # 7 Medgar Evers College Prof. Santos.

BIO 205 – Microbiology Chapters 8, 9, end of Ch. 3.

Growing Microbes L.O: To understand how microorganisms can be safely cultured in a laboratory Answers in your book: Antibiotics do not work on which type.

4-2 Sources of DNA.

Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.

Culturing BacteriaBacteria. Lab Preparation In this lab you will be designing an experiment to test the effectiveness of a product that contains an antibacterial.

Culturing requirements

PREDICTING THE GROWTH OF MICROORGANISMS. Objectives Students will Use sterile techniques to collect a sample Observe the growth of microorganisms on a.

Microbial Growth 1.

Study the effect of industrial antibiotics on microbial growth.

Controlling Bacteria. Antimicrobial Agents (Bactericide) Chemical substances that either kill bacteria or inhibit bacterial growth without harming the.

Bacterial growth:. Bacterial Growth Curve: The schematic growth curve shown below is associated with simplistic conditions known as a batch culture. It.

Do Now— What is personal protective equipment (PPE)?

GK-12 Saturday Workshop December 3, Kirby-Bauer Method The Kirby-Bauer method is the most widely used antibiotic susceptibility test in determining.

Lecture 4 Dr. Dalia M. Mohsen Prof. of Microbiology.

Control of Pathogenic Bacteria. Bacteria spread in various ways: 1. moisture droplets in the air 2. dust 3. direct contact 4. fecal contamination 5. animal.

Sponge Soup Lab.

Experiment Questions Leaf Yeast. For what purpose did you use antiseptic wash solution in the investigation of the growth of leaf yeast on agar plates?

IN THE NAME OF ALLAH ALMIGHTY THE MOST COMPASSIONATE THE MERCIFUL.

Effects of physical, chemical and biological agents on bacteria Experiment 4.

Where can we find bacteria?

Antibiogram realisation on a bacterial strain. Materials avalaible Swab Agar with bacteria Antibiotics disks Mueller Hinton culture medium Deposit plan.

Determination of spectrum of activity of antibiotics.

بسم الله الرحمن الرحيم 140 MICRO LAB 5 : PURIFICATION OF MICROORGANISM.

Recognizing the conditions necessary for microbial growth is vital to disease prevention and treatment.

THE MATH OF BACTERIA, LOGARITHMS By: Jamie log ue.

Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)

Microbial Growth Growth in Batch Culture

Isolation of microorganisms

Unit 1 – Living Cells Topic 2 – Microbes! Types of Microbes

Bacteria Cell Culture and Reproduction

BACTERIAL GROWTH & REPRODUCTION

Starter Decide whether the following statements relate to optical methods or dilution plating: Takes a very long time Uses a colorimeter Requires very.

How do we stop or slow down the problem of antimicrobial resistance?

Lab procedures when handling micro-organisms

Biofilms Microbial communities Form slime or hydrogels

Factors affecting population growth

Microorganisms & Biotechnology

Learning Objectives i Understand the basic aseptic techniques used in culturing organisms. ii Understand the principles and techniques involved in culturing.

النمو والعد البكتيري Microbial growth النمو الجرثومي.

Control of Pathogenic Bacteria

Lab procedures when handling micro-organisms

Tools of the Laboratory Power Point #1: Culturing Microorganisms

The Six “I’s” of Microbiology

ANTIMICROBIAL SUSCEPTIBILITY TESTING

Streaking an Agar Plate For

Microbiology Chapter 3, Quiz #1 Review

Presentation transcript:

Bacterial Cultures *Bacteria grow best in warm, moist, dark areas that contain a lot of food. -When we culture bacteria, we provide them with this environment to grow in via a Petri dish containing nutrient agar. Culture – growing a sample of bacteria in a laboratory. Petri dish – plastic container in which bacterial colonies are grown on nutrient agar. Nutrient agar – gel-like substance that provides bacteria with food, water, and moisture. Is its major food source.

Sterile vs. Contaminated -Before a Petri dish is opened, it is sterile. -As soon as it is exposed to the air, it becomes contaminated. Sterile – no bacteria are present. Contaminated – when unwanted bacteria enters the Petri dish.

Inoculating and Incubating -In order to grow bacteria, we take a sample from an object using a sterile Q-tip, inoculate the Petri dish, and incubate the sample over time. Inoculate – place desired bacteria into Petri dish using a sterile Q-tip. Incubate – place in an area to keep warm.

Streaking Petri Dishes

Bacterial Growth -Bacterial growth can be seen growing on nutrient agar in the form of colonies. Colonies – many bacteria growing close together.

Growth curve of bacteria culture  Lag phase: slow growth slow growth (adjust to environment) (adjust to environment)  Exponential phase: rapid growth rapid growth Stationary phase: Stationary phase: reproduction = death rate reproduction = death rate  Death phase: death rate > reproduction rate death rate > reproduction rate

Zones of Inhibition *Scientists use bacterial colonies to test the effectiveness of new antibiotics and antimicrobial agents such as disinfectants and antiseptics. Zone of Inhibition – a clear region around paper discs that are saturated with an antimicrobial agent on the agar’s surface.