Bio Mini Project The Effects of Different Type of Food source on the Growth rate of Saccharomyces Cerevisiae - Vilasini a/p Karthikesan (16UHF04796) -

Slides:



Advertisements
Similar presentations
Aseptic Technique: Media and Equipment
Advertisements

Practical’s. Practical 1 Learning objectives: By the end of the lesson you should be able to State the similarities and differences between animal, plant.
12 / 04 Friday Learning objective: Discuss how and why we use bacteria and fungi to make food such as yoghurt and beer.
Analysis of Biological System An understanding of biological system can be simplified by analyzing the system at several different levels: Cell level:
Each microorganism has an optimum growth temperature, which below it growth decreases reversibly and above it decreases irreversibly due to degradation.
Group#3, UG-5. Wine  Wine is an alcoholic beverage made from the fermentation of grape juice.  Yeast (Saccharomyces cerevisiae) is mostly used for fermentation.
Sterile technique When handling E. coli and other bacteria it is essential that the live cultures do not become contaminated with other bacteria or fungi.
ASEPTIC TECHNIQUE Removing inoculum from a broth culture
Yeast Population Dynamics Lab
Biology Home learning.
B1.1.2 How our bodies defend themselves against infectious disease
Introduction to Lab Ex. 19: Enumeration of Bacteria
Culture Media Lab 2:.
1. All living things are made up of one or more cells Is yeast unicellular or multicellular?
Bacterial count.
Introduction to Lab 6: Ex. Preparation of Culture Media
Growing Microbes L.O: To understand how microorganisms can be safely cultured in a laboratory Answers in your book: Antibiotics do not work on which type.
Culturing of Bacteria.
4-2 Sources of DNA.
Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.
EXERCISE 20. EFFECT OF TEMPERATURE ON GROWTH OF MICROORGANISMS.
LAB NO 8 LAB NO 8 Environmental Factors Affecting Microbial growth.
Lab 2: Culture Media. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any.
The Effects of Chlorine on Harmful Bacteria Group Leow Shawn Tao (1A413, Leader), Sow Jeng Wei (1O220), Justin Soh (1P111), Soh Yee Kiat (1P127)
Microbial Growth 1.
17.3 Domains and Kingdoms 8(C) Compare characteristics of taxonomic groups, including archaea, bacteria, protists, fungi, plants, and animals.
Do Now  List the steps of the SCIENTIFIC METHOD.
HONEY ANTIMICROBIAL EFFECTS Alicia Grabiec Freeport Senior High School Grade 10.
The POISON Drink. Problem  What type of fruit drink permits the least amount of bacterial growth if left open to the environment?
Results of Sources of Contamination. Fungi Bacteria.
Components Preparation Inoculation
Recording, Organizing, and Analyzing Data. Data Tables Useful for communicating scientific data Used to organize and record observations and measurements.
Lecture 4 Dr. Dalia M. Mohsen Prof. of Microbiology.
Experiment Questions Leaf Yeast. For what purpose did you use antiseptic wash solution in the investigation of the growth of leaf yeast on agar plates?
Science Process Skills
Scientific Method & Processing Skills. Observation Using your senses to study the world.
بسم الله الرحمن الرحيم 140 MICRO LAB 5 : PURIFICATION OF MICROORGANISM.
Grouping Species The broadest category in the classification used by most biologists is the domain. Three domains: Bacteria, Archaea, Eukarya Six kingdoms:
Characteristics of Life Regents Biology Unit 1 “The Living Environment” The living environment is essentially a biology course Biology- the study of.
Agricultural microbiology course
Isolation of microorganisms
Cultivation and Isolation of Bacteria NAS: You’re never too old to streak! 1Lecturer: Mr M. Zivuku.
MINI PROJECT: MICROBIOLOGY TITLE: EFFECT OF TIME OF MICROORGANISMS TO GROW AFTER THE FOOD DROPPED ON THE FLOOR.
By: 1. Teo Boon Boon 2. Khor Mei Ann 3. Ooi Shu YU.
Basic Microbiology and Immunology Practical Course 2016.
Unit 1 – Living Cells Topic 1 – Cells and DNA Topic 2 – Microbes!
Bacterial Count.
Chapter 6, part A Microbial Growth.
GENERAL METHODS OF STUDYING
Increase in number of cells, not cell size Populations Colonies
Micro-organisms understand the role of yeast in the production of beer
BIO : Bioinformatics Lab
Before you start get or make a copy of this Table of results
Prof. Dalia M. mohsen Prof. in Microbiology
Chapter 6, part A Microbial Growth.
Tools of the Laboratory: Methods for Culturing of Microorganisms
النمو والعد البكتيري Microbial growth النمو الجرثومي.
Introduction to Biology
Fungi.
Section 3: Domains and Kingdoms
Experimental Design Graphing Test your knowledge Test your Knowledge 2
Yeast Fermentation Abhinandan Chowdhury.
Bacteria.
Culture Media Lab 2:.
All About Nutrition.
Microorganisms in Air.
Chapter 6, part A Microbial Growth.
Homeostasis & Adaptations
World of Microbes and Study of Yeast Reproduction
<<Enter a Lab Report Title>>
Presentation transcript:

Bio Mini Project The Effects of Different Type of Food source on the Growth rate of Saccharomyces Cerevisiae - Vilasini a/p Karthikesan (16UHF04796) - Joey Tay Ruo Yi (16UHF04659) - Suarnamughy d/o Maran (16UHF01984 )

1.0 Introduction What is yeast ? Yeast is unicellular cells which is a type of bacterial with species name of Saccharomyces Cerevisiae.The yeasts are eukaryotic, single-celled microorganisms classified as the members of fungi kingdom.The reasons for this classification are because it has chitin cell wall and the lipids are made up of ester linkage

1.Yeast with orange juice The orange juice has higher percentage of sugar, low pH value from 3.3 to 4.19 (Journal of Dentistry,2006) and 139 percent of the DV for vitamin C which is an acid.This conditions is more suitable for the growth rate of Saccharomyces Cerevisiae.Thus, it has the higher growth rate of bacteria.

2.Yeast with milk Milk contain 90 percent of water by weight which produce the moisture they need for bacteria growth, high sugar content called lactose which will metabolize the bacteria to provide the energy for bacteria reproduction and also rich in protein which increase the rate of bacteria.

3.Yeast with honey Honey is found in natural form which has very low moisture where only a few amount of bacteria can survive in this conditions,high percentage of fructose sugar which are hygroscopic where they don' t contain water in their natural form and low pH value from 3.26 to 4.48 that kills any bacteria growth (Amina Harris, n.d).

2.0 Materials and Methods

15 ml of orange juice is measured out in measuring cylinder. Poured the solution into universal bottle with the portion of dry yeast close it and leave 30 mins at 37.c. 4 portion of 0.5 g dry yeast is weighed. ↓ ↓

Placed the solution on the solidified nutrient agar contained by petri dish. Hockey stick is used to spread the solution on the nutrient agar and the lid of petri dish is placed back. 2ml of solution is taken out using inoculating loop from the universal bottle. ↓ ↓

Petri dish will be left to incubate for 1-3 days. The experiment is observed and recorded- number of bacteria growth. Above steps are repeated with milk and honey. The experiment was conducted with 3 set of nutrient agar for each food sources. ↓ ↓

3.0 Expected Results Manipulated variable : Different type of food sources Responding variable : Growth rate of yeast Constant variable : The temperature of food sources, Amount of Saccharomyces Cerevisiae, Volume of food sources

Table 1: Average number of bacteria produced with different types of food source. Experimental Set ContentsAverage Number of Bacteria found in 1 cm 3 Final Number of Bacteria produced in Petri Dish Yeast with nutrient agar Yeast with orange juice Yeast with milk Yeast with honey