Shimura Supplemental Figure 1

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Shimura Supplemental Figure 1 HepG2 HeLa Cell Number (x105) Day

Shimura Supplemental Figure 2 HeLa % of apoptotic cells Dose of X-ray (Gy)

Time after irradiation (hour) Shimura Supplemental Figure 3 A B 10 Gy 12 hours 10 Gy 24 hours 31FR G2-phase (37.02%) G2-phase (68.58%) BrdU G2-phase fraction (%) 31FR-31NR G2-phase (32.09%) G2-phase (75.48%) Time after irradiation (hour) DNA content

Shimura Supplemental Figure 4 C HeLa 0FR 31FR 0FR 31FR IR (Gy) 0 10 0 10 Cyclin D1 Cdk4 HE Actin B Cyclin D1 Tumor Volume (mm3) Day

Shimura Supplemental Figure 5 Cdk4 CCND1 Cdk2 Level of Expression 0FR 14FR 31FR 45FR 0FR 14FR 31FR 45FR 0FR 14FR 31FR 45FR

Shimura Supplemental Figure 6 10-Gy Irradiation 0FR 31FR After CHX (minutes) 0 10 20 30 60 90 0 10 20 30 60 90 Cyclin D1 Actin 10-Gy Irradiation Relative cyclin D1 levels Time after CHX (min)

Shimura Supplemental Figure 7 HepG2 HeLa 2Gy 0.5Gy 2Gy 0.5Gy Time after IR (hr) 0 0.5 1 3 6 24 24 0 0.5 1 3 6 24 24 P-AKT (Ser473) AKT Actin B HepG2 HeLa 2Gy 0.5Gy 2Gy 0.5Gy Time after IR (hr) 0 0.5 1 3 6 24 24 0 0.5 1 3 6 24 24 P-GSK3b (Ser9) GSK3b Actin

Shimura Supplemental Figure 8 HepG2 HeLa 0FR 31FR-31NR 0FR 31FR-31NR KU-55933 - + - + - + - + P-ATM P-AKT (Ser473) AKT Actin

Shimura Supplemental Figure 9 0FR 31FR-31NR HepG2 tail HeLa tail