Chem 51LB Week 4 2016 Summer Session 1 Kevin Chen.

Slides:



Advertisements
Similar presentations
Gas Chromatography.
Advertisements

Synthesis and Analysis of Aspirin Chemistry 1060 Laboratory.
Chromatography CHEMISTRY Chromatography Chromatography is a technique for separating species based on physical or chemical properties. Usually.
In carbon-13 NMR, what do the number of peaks represent?
Dehydration of 2-Butanol and Dehydrobromination of 2-Bromobutane;
Chem 334 Organic Chemistry Laboratory I 334-FPBP and
Today: Exp.9, “Separation and Identification of an Unknown Mixture” (3 lab periods) “Qualitative analysis” This experiment includes: liquid/liquid.
Synthesis of Oil of Wintergreen
Distillation and Chromatography. Objectives Separate the components of a solution using distillation and chromatography Determine physical or chemical.
What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.
What is Chromatography? Derived from the Greek word Chroma meaning colour, chromatography provides a way to identify unknown compounds and separate.
Chromatography Year 12.
PARTITION CHROMATOGRAPHY
By, Blessy Babu. What is Gas Chromatography?  Gas spectroscopy is a technique used to separate volatile components in a mixture.  It is particularly.
Chemical Analysis. Analytical Techniques When chemical evidence is collected at a crime scene, it must be run through an instrument. These instruments.
Chromatography. TLC Thin layer chromatography Stationary phase Mobile phase.
Chromatography Tomáš Mlčoch Pavel Moťka. Chromatography Described by Tswett in 1906 Described by Tswett in 1906 He separated some pigments using a tube.
Separation Science Differences in IMFs can be used to separate chemical substances for further analysis. Differences in IMFs can be used to separate chemical.
Alexandria University Faculty Of Science Practical Training Course In Applications Of Plant Physiology Prepared By Dr. Mohamed Mohamed Ibrahim Lecturer.
Lecture 12 Chromatography Introduction Ch 7: Thin-Layer Chromatography Lecture Problem 4 Due This Week In Lab: Ch 6: Procedures 2 & 3 Due: Ch 5 Final Report.
CHEMISTRY 1000 Topic #2: The Chemical Alphabet Spring 2013 Dr. Susan Findlay Gallium, Ga Sodium, Na Forms of Carbon.
Gas Chromatography Experiment. Gas Chromatography - Gas Chromatography (GC) is a common technique used to separate and identify volatile organic compounds.
COLUMN CHROMATOGRAPHY (CC). TLC - Optimizing for column chromatography Optimum: 0.2 < R f < 0.5.
Metabolomics and analytical chemistry: GC & GCMS Simone Bossi Analytical Chemistry Lab – Plant Physiology – Plant Biology.
Methods of Separating Mixtures
Recrystallization and Melting Point
Chromatography Homogeneous mixtures of a solvent and one or more solutes (dissolved substances) are often separated by chromatography. Chromatography works.
Lab Activity 7 IUG, Fall 2012 Dr. Tarek Zaida IUG, Fall 2012 Dr. Tarek Zaida 1.
Gas Chromatography An Introduction. What is it? Gas chromatography – (gas-liquid chromatography) involves a sample being vaporized and injected onto the.
SEPARATING MIXTURES Chapter 3. SEPARATING MIXTURES Because substances in a mixture are physically combined, the processes used to separate a mixture are.
EQ: What are the different methods of physical separation? SEPARATION OF MATTER NOTES.
1 Classification and purification of Organic Compounds.
Experiment 4: TLC and HPLC of Nitroanilines. Objectives  To learn the separation techniques of Thin Layer Chromatography and HPLC chromatography.  To.
Chromatography.
Drug detection and analysis
Experiment 5: Chromatography
Biochemical instrumental analysis - 9 Dr. Maha Al-Sedik 2016 CLS 332.
THIN LAYER CHROMATOGRAPHY.
Chem 51LB Week Summer Session 1 Kevin Chen.
Chem 51LB Week Summer Session 1 Kevin Chen.
Chem 51LB Week Summer Session 1 Kevin Chen.
Separating Mixtures Differences in physical properties can be used to separate mixtures What are some physical properties we can use to separate mixtures?
From the Greek for “color writing”
Chemsheets AS006 (Electron arrangement)
Organic Chemistry Lab 315 Fall, 2016.
Chemical Ideas 7.6 Chromatography.
2016 Summer Session 1 Kevin Chen
LU 3: Separation Techniques
Lab Activity 7 Separation of blood serum lipids by thin-layer chromatography IUG, Dr. Tarek Zaida.
Lab Activity 5 Separation of blood serum lipids by thin-layer chromatography IUG, Fall 2017 Dr. Tarek Zaida.
Lab Activity 4 IUG, Fall 2017 Dr. Tarek Zaida.
What is Chromatography?
Organic Instrumentation
Option A Part 3: AA and Chromatography
SEPARATION TECHNIQUES
Concentration: the amount of substance in a certain space
Lab Activity 7 Separation of blood serum lipids by thin-layer chromatography IUG, Fall 2017 Dr. Tarek Zaida.
What is chromatography?
Lab Activity 4 IUG, Fall 2017 Dr. Tarek Zaida.
Chromatography Daheeya Alenazi.
Separation Techniques
Paper title-Analytical techniques in chemistry
What is Chromatography?
Unit 5: Drugs 5-4 Forensic Drug Analysis Part 1 May 24, 2019
What is Chromatography?
Colligative Properties
Introduction and chromatography
5 week plan Each week you will have 2 single lessons and 1 double lesson. In those lessons you will learn aspects from Modern Analytical Techniques (MAT)
GAS CHROMATOGRAPHY.
Presentation transcript:

Chem 51LB Week Summer Session 1 Kevin Chen

Lecture and lab participation Dr. Borovik’s unique participation method… clapping

Lecture and lab participation Dr. Borovik’s unique participation method… clapping Who wants to get an A in this class?

Lecture and lab participation Dr. Borovik’s unique participation method… clapping Who wants to get an A in this class? Who will obey all safety rules in lab?

Lecture and lab participation Dr. Borovik’s unique participation method… clapping Who wants to get an A in this class? Who will obey all safety rules in lab? Who will write great lab reports and learn a lot and make me proud?

Experiment #5: Acid-catalyzed dehydration and base-catalyzed debromination Given: primary and secondary alcohol for acid- catalyzed dehydration and primary and secondary alkylbromides for base-catalyzed debromination (4 compounds total!) Goal: Setup and run reaction and use gas chromatography (GC) for anlaysis End game: identify stereo- and regio- chemistry of the reaction products, and compare E1 versus E2 Note : Make sure to get data from another group

Part A: acid-catalyzed dehyrdation Reactants: 1-butanol and 2-butanol Catalyst: concentrated sulfuric acid Products(s): 1-butene, trans-2-butene, cis-2- butene GC for analysis – Identification – Mixture(?) components aka product distribution

Part A: Setup Product is a gas! Sealed reaction vessel is DANGEROUS!

Gas Chromatography Separation technique of gaseous mixture based on differences in boiling points – Lower boiling point = more volatile = moves faster – Higher boiling point = less volatile = moves slower Two phases – Solid phase (really, really long capillary column) – Mobile phase (inert gas: helium or nitrogen) Similarity to TLC (based on polarity)

~0.1mm thin, and up to 100m long coiled inside an oven Can be packed

Separation technique comparisons Gas chromatographyLiquid chromatography (TLC) SampleGas and liquid (must vaporize) Liquid and solid (must dissolve) Separation is based on...Boiling pointPolarity Mobile phaseInert gasMixture of polar and nonpolar solvents (overall nonpolar) Stationary phaseLong copper columnSilica (polar) Separation methodTemperaturePolarity of mobile phase Can you use this to identify your compound? Yes! How?Compare to standards!

Separation technique comparisons Gas chromatographyLiquid chromatography SeparatesGas and liquid (must vaporize) Liquid and solid (must dissolve) Separation is based on...Boiling pointPolarity Mobile phaseInert gasMixture of polar and nonpolar solvents (overall nonpolar) Stationary phaseLong copper columnSilica (polar) Separation methodTemperaturePolarity of mobile phase Can you use this to identify your compound? Yes! How?Compare to standards!

Part 2: base-catalyzed debromination Reactants: 1-bromobutane and 2-bromobutane Catalyst: 1M tBuOK Products(s): 1-butene, trans-2-butene, cis-2- butene GC for analysis – Identification – Mixture(?) components aka product distribution

Looks familiar? It should! Note: need a water condenser

Experiment #6: Separation and identification of unknown mixture Given: mixture of unknown (2 compounds from 9 possible options) In lab goal: You decide! End game: separate the unknowns and identify them using ALL relevant spectroscopy data Note: Must research structures and properties before first lab! PENALTY… NO LAB!

Experiment #6: Separation and identification of unknown mixture Given: unknown mixture (2 compounds from 9 possible options) In lab goal: You decide! End game: separate the major component of the unknown mixture and identify BOTH unknowns using ALL relevant spectroscopy data Note: Must research structures and properties before first lab! PENALTY… NO LAB!

My game plan (before lab) RESEARCH! Only 9 possible compounds (but 72 different combinations) – Identify structures – Group them based on similarity Why is this useful? NMR! Properties – Are they Solid, liquid, or gas? Color? Why is this useful? Comparing physical property of pure, isolated sample – Are they acid, base, or neutral? Why is this useful? Acid/base extraction

Techniques at your disposal Melting point – mix melting point Thin layer chromatography – With standards (Rf) Recrystallization Acid/base separation NMR IR GC

My game plan (1 st lab) Identify unknowns – NMR – Perform simple experiments to confirm TLC and melting point Must develop a plan to separate mixture for 2 nd lab Start separation if possible (likely not)

Teaser for next week! Finish experiment #6 Practicals – Review next week in lecture