From: Mechanisms of Murine Lacrimal Gland Repair after Experimentally Induced Inflammation Invest. Ophthalmol. Vis. Sci.. 2008;49(10):4399-4406. doi:10.1167/iovs.08-1730 Figure Legend: Involvement of autophagy and the lysosomal compartment in acinar cell death. (A) Lacrimal glands removed from control (C), saline-treated (S), or IL-1–treated animals were processed for Western blotting using an antibody against LAMP-1. Data in the plot are mean ± SEM from three independent experiments. *Statistically significant difference from control. #Statistically significant difference from saline. (B) Lacrimal glands removed from saline- or IL-1–treated (3-day) animals were processed for double-immunofluorescence studies using the LAMP-1 antibody (green) and an antibody against MAP LC3, a marker of autophagic vacuoles (red). Nuclei were counterstained with DAPI (blue). Arrowheads and inset highlight cells harboring LAMP-1 and MAP LC3 immunoreactivity. Scale bar, 50 μm. Date of download: 9/18/2016 The Association for Research in Vision and Ophthalmology Copyright © 2016. All rights reserved.