EcoRI HindIII M M bp 23130 4361 2027 564 Fig. S1A: Comparison of restriction patterns of genomic DNAs isolated from TP-J34, TP-778L and TP-DSM-20617. The.

Slides:

Advertisements

Similar presentations

RESTRICTION ENZYMES & GEL ELECTROPHORESIS ANALYSIS OF PRECUT LAMBDA DNA.

Advertisements

A Timed Presentation - do not click the mouse. Approximate Run Time – 4 minutes and 45 seconds.

Ch 9 Frontiers of Biotechnology

LAB SIX Molecular Biology. Three samples of phage lambda DNA: 1.Digested with EcoRI 2.Digested with HindIII (fragments are of known size) 3.Undigested.

Plasmids and Restriction Enzyme Mapping

Southern Transfer. Research Plan Isolate Genomic DNA Southern Blot Analysis Digest Genomic DNA w/ Various Restriction Enzymes Agarose Gel Electrophoresis.

CAPS Cleaved Amplified Polymorphic Sequence. CAPS - CAPS (cleaved amplified polymorphic sequence) is also known as PCR-RFLPs (polymerase chain reaction-restriction.

What I Learned about Southern Blotting By: Matthew Garma (LCC) A.B.E. Workshop 2006.

Southern Transfer. Research Plan Isolate Genomic DNA Digest Genomic DNA with Various Restriction Enzymes Agarose Gel Electrophoresis and Southern Transfer.

Restriction Enzymes.

Lecture 19, Chapter 11 Analysis of transgenic plants part II Neal Stewart.

Restriction mapping revision

Big Idea 3 – Investigation (Lab) 9 We did a variation of this lab at the Cold Spring Harbor DNA Learning Center West… The next few slides highlight the.

Analysis of Transgenic Plants. 1.Regeneration on Selective Medium Selectable Marker Gene.

Restriction Digestion and Analysis of Lambda DNA Kit

Gel Electrophoresis Lab

TOPICS IN (NANO) BIOTECHNOLOGY Lecture 7 5th May, 2006 PhD Course.

Silly Lab Tech Which plasmid is it??. Your mission… Your silly lab tech did not label tubes of purified plasmid DNA that you need for your experiments!!

Molecular Genetics Lab Review. Bacterial Transformation Genetic transformation—host organism takes in and expresses foreign DNA Genetic engineering—manipulation.

Manipulation of DNA. Restriction enzymes are used to cut DNA into smaller fragments. Different restriction enzymes recognize and cut different DNA sequences.

Revision – Concept map.

(RFLP Electrophoresis)

Treatments that stimulate the E.coli. to take up foreign plasmids include: 1.CaCL2 treatment 2.Heat shock 3.Incubation with ECORI 4.1 and and 3 6.All.

A Timed Presentation - do not click the mouse. Approximate Run Time - 2 minutes 38 seconds.

Objectives: Introduce the students to digest genomic DNA by restriction endonucleases. Observe the results of digestion on agarose gel electrophoresis.

LAB 6 DNA FINGERPRINTING. BUILD the GEL FRAME Position comb and lock in place in side slots.

A 2 nd year college student working in the lab for the summer forgot to label the Tube of plasmid DNA that he/she was given. So being the brilliant graduate.

AIM: Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning.

Recognition sequences Restriction enzyme EcoRI cuts the DNA into fragments. Sticky end.

RESTRICTION ENZYMES & GEL ELECTROPHORESIS FORENSIC DNA FINGERPRINTING.

Electrophoresis is a molecular technique that separates nucleic acids and proteins based on Size and +-+ Charge +-+

Restriction Enzymes. Discovery  In 1962, Werner Arber, a Swiss biochemist, provided the first evidence for the existence of "molecular scissors" that.

Fig. S1: Fingerprinting of three BAC clones from different accessions of wild rice species with the AA genome constitution. The BAC DNAs were completely.

Restriction Enzyme Digestion of Phage DNA

Midterm Breakdown Part I Part II: Part III : 8 calculations

Chapter 7 Recombinant DNA Technology and Genomics

RE Digestion Field Trip Results

Restriction Enzymes-BIOL 202

Restriction Enzyme Analysis of Lambda DNA

Restriction Enzyme Analysis of Lambda DNA

Restriction Digestion and Analysis of Lambda DNA Kit

Restriction Digestion and Analysis of Lambda DNA Kit

Molecular Biology Restriction enzymes.

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

Volume 8, Issue 2, Pages (February 2001)

Isolation and characterisation of toxin A-negative, toxin B-positive Clostridium difficile in Dublin, Ireland D. Drudy, N. Harnedy, S. Fanning, R. O'Mahony,

Simulating Genetic Screening

High rates of antibiotic resistance among normal fecal flora Escherichia coli isolates in children from Greece A.C. Vatopoulos, E. Varvaresou, E. Petridou,

RE Digestion Field Trip Results

Temporal expression of the transgenic human protamine gene cluster

Cloning a DNA segment from bacteriophage

Restriction Enzymes-BIOL 202

S. Vourli, H. Tsorlini, H. Katsifa, M. Polemis, L. S. Tzouvelekis, A

Linear Mitochondrial Plasmids of F

Molecular Biotechnology

S.B. Schwartz, K.A. Thurman, S.L. Mitchell, B.J. Wolff, J.M. Winchell

Induction of Large DNA Palindrome Formation in Yeast: Implications for Gene Amplification and Genome Stability in Eukaryotes David K Butler, Lauren E.

Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical.

9-3 DNA Typing with Tandem Repeats

Restriction Digestion and Analysis of Lambda DNA Kit

Comparison of macrorestriction analysis of genomic DNA by pulsed-field gel electrophoresis and ribotyping with conventional methods for differentiation.

Clonal dissemination of two clusters of Acinetobacter baumannii producing OXA-23 or OXA-58 in Rome, Italy R.E. Mendes, T. Spanu, L. Deshpande, M. Castanheira,

Effect of Genome Size on AAV Vector Packaging

DNA fingerprinting (genetic fingerprint)

Molecular Biotechnology 4C x MWM

A family outbreak due to an emm-type 11 multiresistant strain of Streptococcus pyogenes C. Martinaud, A. Doloy, B. Graffin, T. Gaillard, R. Poyet, S.

Association of a polymorphism in the ornithine decarboxylase gene with male androgenetic alopecia Rachel A. Garton, MD, Amy J. McMichael, MD, Joel Sugarman,

Supplementary Fig. 1 bp E F G H I Hybrid 1 SP2/0 MW P3X (-)

Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. Integration of the kDNA minicircle sequence into the genome.

Presentation transcript:

EcoRI HindIII M M bp Fig. S1A: Comparison of restriction patterns of genomic DNAs isolated from TP-J34, TP-778L and TP-DSM The restriction digests were separated on 0.8 % agarose gels. M = bp marker (HindIII-digested Lambda DNA). A)

MW+14: HindIII-verd. λ-DNA 1: TP-J34 (temperent) 2: TP-778L (temperent) 3: Sfi21 (temperent) 4: O1205 (temperent) 5: 858 (virulent) 6: 2972 (virulent) 7: 5093 (virulent) 8: 7201 (virulent) 9: Sfi11 (virulent) 10: Sfi19 (virulent) 11: Abc2 (virulent) 12: ALQ13.2 (virulent) 13: DT-1 (virulent) MW B) Fig. S1B: Comparison of HindIII restriction patterns of genomic DNA isolated from TP-DSM with in silico-generated HindIII patterns of different Streptococcus phages’ genomic DNAs. To the right, the restriction digest of genomic TP-DSM20617 DNA separated on 0.8 % agarose gel is shown together with marker DNA. MW = bp marker (HindIII-digested Lambda DNA).

MW+14: HindIII-verd. λ-DNA 1: TP-J34 (temperent) 2: TP-778L (temperent) 3: Sfi21 (temperent) 4: O1205 (temperent) 5: 858 (virulent) 6: 2972 (virulent) 7: 5093 (virulent) 8: 7201 (virulent) 9: Sfi11 (virulent) 10: Sfi19 (virulent) 11: Abc2 (virulent) 12: ALQ13.2 (virulent) 13: DT-1 (virulent) MW C) Fig. S1C: Comparison of EcoRI restriction patterns of genomic DNA isolated from TP-DSM with in silico-generated EcoRI patterns of different Streptococcus phages’ genomic DNAs. To the right, the restriction digest of genomic TP-DSM20617 DNA separated on 0.8 % agarose gel is shown together with marker DNA. MW = bp marker (HindIII-digested Lambda DNA).