Intraplatelet Tetrahydrobiopterin Plays an Important Role in Regulating Canine Coronary Arterial Thrombosis by Modulating Intraplatelet Nitric Oxide and Superoxide Generation by Seiji Kanaya, Hisao Ikeda, Nobuya Haramaki, Toyoaki Murohara, and Tsutomu Imaizumi Circulation Volume 104(20): November 13, 2001 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Representative traces showing effects of saline, BH4, and BH4+L-NMMA on phasic left anterior descending (LAD) coronary flow pattern. Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. Ex vivo platelet aggregation in response to various concentrations of ADP and U46619 of platelets obtained at baseline (□), during CFVs (○), and after treatment (). *P<0.01 vs baseline (before CFVs), †P<0.05 vs during CFVs (before treatment). Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Intraplatelet BH4 levels at baseline, during CFVs, and after treatments. Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Platelet P-selectin expression at baseline, during CFVs, and after treatments. Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Intraplatelet cGMP levels at baseline, during CFVs, after administration of BH4, and after administration of BH4+L-NMMA. Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.

Figure 6. Left, Representative histograms of intraplatelet nitrotyrosine production at baseline, during CFVs, and after treatment with BH4 30 mg/kg. Seiji Kanaya et al. Circulation. 2001;104: Copyright © American Heart Association, Inc. All rights reserved.