Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme Heating Substrate Cooling Enzyme Cooling Substrate Concentration Increasing enzyme conc Increasing substrate conc

Enzyme denatures in heat Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Cooling Enzyme Cooling Substrate Increasing enzyme conc Increasing substrate conc

Enzyme denatures in heat Decreases More Molecular Kinetic Energy Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Decreases More Molecular Kinetic Energy  Cooling Enzyme Cooling Substrate Concentration Increasing enzyme conc Increasing substrate conc

Enzyme denatures in heat Decreases More Molecular Kinetic Energy Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Decreases More Molecular Kinetic Energy  Cooling Enzyme Increases   Less Molecular Kinetic Energy Cooling Substrate Concentration Increasing enzyme conc Increasing substrate conc

Enzyme denatures in heat Decreases More Molecular Kinetic Energy Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Decreases More Molecular Kinetic Energy  Cooling Enzyme Increases   Less Molecular Kinetic Energy Cooling Substrate  Increases Concentration Increasing enzyme conc Increasing substrate conc

Enzyme denatures in heat Decreases More Molecular Kinetic Energy Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Decreases More Molecular Kinetic Energy  Cooling Enzyme Increases   Less Molecular Kinetic Energy Cooling Substrate  Increases Concentration Increasing enzyme conc Decreases  Assuming there is enough substrate; more enzyme increase the number of collisions and speeds up the reaction  Increasing substrate conc

Enzyme denatures in heat Decreases More Molecular Kinetic Energy Variable Reaction Time… Why does this Happen? Temperature   Heating Enzyme  STOPS Enzyme denatures in heat  Heating Substrate Decreases More Molecular Kinetic Energy  Cooling Enzyme Increases   Less Molecular Kinetic Energy Cooling Substrate  Increases Concentration Increasing enzyme conc Decreases  Assuming there is enough substrate; more enzyme increase the number of collisions and speeds up the reaction  Increasing substrate conc  Decreases More substrate (H2O2) increases the number of collisions until the point in which the enzyme is saturated