Measuring Concentration Flame Photometer

Using the Flame Photometer Spectroscopy: Study of light produce by a substance Spectroscopy is a common form of chemical analysis it is based on a principle of the Bohr model: Atoms absorb and release specific wavelengths (colours) of light because of the E.L. they possess

Emission Spectra Element X The emission spectrum of each element is “fingerprint” of that element When excited, a sample will betray the presence of a substance by its emission spectrum: Element X

Emission Spectra Concept 1 A sample that contains the matching spectral lines compared to a standard must contain that element Sample Spectrum (This sample contains element X) Standard containing Element X

Emission Spectra Concept 2 The concentration of an element is a sample is proportional to the brightness of the spectrum Sample Spectrum 1 Std 2 Element X is more concentrated in sample #2

Flame Photometer Device measures the concentration of a element in a sample by measuring the intensity of its’ emission spectrum Parts Pump Burner Nebulizer Photodetector readout ! 0.000

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.000 Zero with distilled water std1 std2 std 3 ukn c=2g/L c=4g/L c=6g/L c=? ! 0.000

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.010 Zero with distilled water Measure the concentration of several standard solutions, each more concentrated than the next (0.010) std1 std2 std 3 ukn e=0.010 c=2g/L c=4g/L c=6g/L c=? ! 0.010 Conc = 1 g/L

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.020 Zero with distilled water Measure the concentration of several standard solutions, each more concentrated than the next (0.010, 0.020) std1 std2 std 3 ukn e=0.010 e=0.020 c=2g/L c=4g/L c=6g/L c=? ! 0.020 Conc = 4 g/L

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.030 Zero with distilled water Measure the concentration of several standard solutions, each more concentrated than the next (0.010, 0.020, 0.030 std1 std2 std 3 ukn e=0.010 e=0.020 e=0.030 c=2g/L c=4g/L c=6g/L c=? ! 0.030 Conc = 6 g/L

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.026 Zero with distilled water Measure the concentration of several standard solutions, each more concentrated than the next (0.010, 0.020, 0.030) Measure unknown (0.026) std1 std2 std 3 ukn e=0.010 e=0.020 e=0.030 e=0.026 c=2g/L c=4g/L c=6g/L c=? ! 0.026 Conc = ?

Pocedure To use a flame photometer std1 std2 std 3 ukn ! 0.000 Zero with distilled water Measure the concentration of several standard solutions, each more concentrated than the next (0.010, 0.020, 0.030) Measure unknown (0.026) Compare unkn to stds using calibration curve ! std1 std2 std 3 ukn e=0.010 e=0.020 e=0.030 e=0.026 c=2g/L c=4g/L c=6g/L c=? 0.000 Conc = ?

Conc of standards (g/L) std1 std2 std 3 ukn c=2g/L c=4g/L c=6g/L c=? e=0.010 e=0.020 e=0.030 e=0.026 Calibration Curve Ukn emission 0.026 .04 .03 Emission Of standards .02 Conc of unknown = 5.2 g/L .01 2 4 6 8 Conc of standards (g/L)