Evidence That Estrogen Suppresses Rho-Kinase Function in the Cerebral Circulation In Vivo by Sophocles Chrissobolis, Klaudia Budzyn, Philip D. Marley, and Christopher G. Sobey Stroke Volume 35(9): September 1, 2004 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. a, Vasodilatation elicited by the Rho-kinase inhibitor Y in basilar artery of male (n=6; baseline diameter= 230±10 μm) and female (n=8; baseline diameter=234±21 μm) rats in vivo. *P<0.05 vs females. b, Vasodilatation by acetylcholine (ACh) in males (n=5; baseline diameter=223±11 μm) and females (n=12; baseline diameter=236±13 μm). c, Vasodilatation by aprikalim in males (n=5; baseline diameter=231±10 μm) and females (n=5; baseline diameter=208±17 μm). Sophocles Chrissobolis et al. Stroke. 2004;35: Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. a, Change in basilar artery diameter of male (n=14; baseline diameter=253±9 μm) and female (n=24; baseline diameter= 252±5 μm) rats in response to L-NAME (1 mmol/L). b, Change in basilar artery diameter of males (n=6; baseline diameter= 249±18 μm) and females (n=5; baseline diameter=269±11 μm) in response to serotonin (0.1 μmol/L). *P<0.05 vs male. Sophocles Chrissobolis et al. Stroke. 2004;35: Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. a, Vasodilatation by Y in basilar artery of OVX rats treated with vehicle (Veh) (n=6; baseline diameter= 258±17 μm) and OVX rats treated with 17β-estradiol (E2) (n=6; baseline diameter=230±12 μm). Sophocles Chrissobolis et al. Stroke. 2004;35: Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. a, Change in basilar artery diameter of OVX rats treated with vehicle (Veh) (n=7; baseline diameter=269±14 μm) and OVX rats treated with 17β-estradiol (E2) (n=5; baseline diameter=255±9 μm) in response to L-NAME (1 mmol/L). b, Change in basilar artery diameter of OVX rats treated with vehicle (n=6; baseline diameter=265±17 μm) and OVX rats treated with E2 (n=4; baseline diameter=252±13 μm) in response to serotonin (0.1 μmol/L). Sophocles Chrissobolis et al. Stroke. 2004;35: Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. a, Top, Representative Western blot showing RhoA expression in basilar artery of male (n=6) and female (n=5) rats. Sophocles Chrissobolis et al. Stroke. 2004;35: Copyright © American Heart Association, Inc. All rights reserved.