AP EXAM REVIEW SESSION MOLECULAR GENETICS Be prepared, not scared!

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AP EXAM REVIEW SESSION MOLECULAR GENETICS Be prepared, not scared!

Nucleic Acids

DNA

DNA Replication

Mitosis vs. Meiosis

Mitosis vs. Meiosis, cont

Meiosis in Humans

Mechanisms of Gene Transfer in Bacteria

Gene Expression DNA → Protein

Prokaryotic Regulation of Gene Expression Operons

Eukaryotic Gene Expression

REGULATION OF EUK GENE EXPRESSION Pre-Transcriptional Modifications  Chromatin Re-modeling o Acetylation of Histones o Methylation  Initiation of Transcription o Transcription Factors

REGULATION OF EUK GENE EXPRESSION Post-Transcriptional Modifications, cont  mRNA Degradation  Protein Processing o Proteosomes  Non-Coding RNAs o microRNAs (miRNAs) o small interfering RNAs (siRNAs)

TECHNIQUES IN MOLECULAR GENETICS Restriction Enzymes & Recombinant DNA

TECHNIQUES IN MOLECULAR GENETICS Gene Cloning

TECHNIQUES IN MOLECULAR GENETICS Using mRNA to Produce cDNA

TECHNIQUES IN MOLECULAR GENETICS Microarrays

TECHNIQUES IN MOLECULAR GENETICS Polymerase Chain Reaction (PCR)

TECHNIQUES IN MOLECULAR GENETICS Gel Electrophoresis

TECHNIQUES IN MOLECULAR GENETICS Restriction Fragment Length Polymorphisms, AKA... RFLPs

TECHNIQUES IN MOLECULAR GENETICS Single Nucleotide Polymorphisms - SNPs