Arginase I enhances vascular smooth muscle cell proliferation by increasing intracellular polyamine production (A and B) Arginase I enhances cell proliferation.

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Arginase I enhances vascular smooth muscle cell proliferation by increasing intracellular polyamine production (A and B) Arginase I enhances cell proliferation in vivo. Arginase I enhances vascular smooth muscle cell proliferation by increasing intracellular polyamine production (A and B) Arginase I enhances cell proliferation in vivo. (A) Representative immunohistochemical images for proliferating cell nuclear antigen and (B) quantification. Data are means ± SD; n = 10 rabbits per group. (C and D) Arginase I promotes human aortic smooth muscle cell proliferation in vitro. Transfected human aortic smooth muscle cells were incubated at 37°C for 72 h in complete SMC medium containing 0.4 mM l-arginine. Cell proliferation was assessed by EdU incorporation after a 3-day (72 h) growth. BEC was added to cells at Day 2 (24 h). (C) Representative images of EdU incorporation (red) and (D) quantification. Data are means ± SD from five independent microscope fields for each of three independent experiments. (E) Content of polyamines in human aortic smooth muscle cells incubated with 0.4 mM l-arginine. Data are means ± SD of duplicate determinations from three independent experiments. Xu-Ping Wang et al. Eur Heart J 2014;35:911-919 Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2013. For permissions please email: journals.permissions@oup.com