Protein Synthesis and the Genetic Code II 25 October 2013.

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Protein Synthesis and the Genetic Code II 25 October 2013

The three steps of translation Initiation Elongation Termination

Reminder: Ribosome structure

Initiation - Prokaryotes

Polycistronic mRNA in bacteria Shine-Dalgarno sequence: AGGAGGU

Initiation - Prokaryotes

Initiation and addition of 2 nd AA

Initiation - eukaryotes

Elongation Cycle

Peptide Bond Formation

Catalytic site on the rRNA

Elongation Cycle

Role of EF-Tu

Role of EF-G

Termination RRF: ribosome release factor

Termination in more detail RRF: ribosome release factor

Translational recoding

Translational frameshift

Antibiotics and Translation Tetracyclin : blocks binding of aminoacyl- tRNA to the ribosome. Chloramphenicol : Inhibits peptidyl- transferase activity of the ribosome Erythromycin : Blocks ribosome translocation movements.

Puromycin resembles an aminoacyl-tRNA

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