Silhouette Sculptor for men and women

UNISLIM  : 2-way activity Lipolysis Activation Decrease of lipid entry Phosphodiesterase inhibition  Presence of lipid receptors on the cell membrane DESTOCKING BLOCKS THE LIPID STORAGE Green coffeeYerba Mate

UNISLIM  Complex of 2 plant extracts Green coffee (Cafestol + Kahweol) Yerba Mate (Methyl-xanthine)

Lipolysis : Phosphodiesterase inhibition  ATP AMP + Phosphodiesterase Protein kinase Cyclic AMP Fatty acids Glycerol Triglycerides ADIPOCYTES Adényl cyclase Triglyceride lipase UNISLIM (Methyl-xanthine) STOP SKIN Inactive ACTIVE

Efficacy in vitro Lipolytic effect 3% of UNISLIM  increases the reaction : % adipocytes UNISLIM 3 hours Assay Triglyceride  Fatty acids + Glycerol

Mechanism of the lipids integration in the adipocyte Food Transport of triglycerides in the blood mainly by the Low Density Lipoproteins (LDL) and the Very Low Density Lipoproteins (VLDL) The lipoproteins are integrated in the adipocytes via LDL and VLDL receptors Nobel prize, Goldstein (1990), demonstrates that free cholesterol in the adipocyte is a “signal” that inhibits the synthesis of lipoprotein receptors.

Regulation of the lipid entry: Beyond a threshold of cholesterol in the adipocyte, the synthesis of LDL and VLDL receptors is retro-regulated (inhibition). LDL Nucleus (DNA) Lysosome DIGESTION Receptor synthesis Adipocyte LDL receptor VLDL receptor VLD L LDL ENDOCYTOSIS VLD L Triglycerides – – Cholesterol – – –

CAFESTOLKAHWEOL CAFESTOL and KAHWEOL from Green Coffee are structural analogues of cholesterol. They mimic the effect of cholesterol. They inhibit the synthesis of lipid receptors, then the integration in the adipocyte is limited.

LDL Nucleus (DNA) Lysosome DIGESTION Receptor synthesis Adipocyte LDL receptor VLDL receptor VLD L LDL ENDOCYTOSIS VLD L Triglycerides – – Cholesterol – – – – CAFESTOL KAHWEOL

Decrease of the expression of LDL and VLDL receptors Quantification of RNAm involved in the synthesis of LDL and VLDL receptors (DNA array) Control Variation Green coffee extract 0.45x10 -3 % (Equivalent to 15ppm cafestol acetate) RNAm expression VLDL receptorLDL receptor %-33% 8767 The number of VLDL and LDL is decreased. The entry of triglycerides in the adipocyte is lessened.

Accelerates the destocking of the lipids + Minimizes the entry of the lipids = Reduction of the adipocyte size

Efficacy in vivo 12 Female volunteers Formula containing 3% UNISLIM  2 months, Twice daily application Analysis by topometry (fringe technique)

Topometry (fringe technique) The software measures the distance between the surface of the zone before treatment and the surface of the zone after treatment

Fringe technique

Thighs’ volume Average reduction: Very significant (p<0.01) – 10% Up to -27% – 10% Up to -27%

Average reduction : very significant (p<0.01) Thighs’ perimeter – 0,8 cm Up to -1,9 cm – 0,8 cm Up to -1,9 cm

15 Male volunteers Formula containing 3% UNISLIM TM 2 months, Twice daily application Analysis by topometry (fringe technique) Efficacy in vivo

Volume of folds of fat -17,7 cm 3

UNISLIM  Cosmetic liposuction