Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain.

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Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain

Introduction Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Kinetic range Thermodynamic range Extraction Distribution constant Temperature Ionic strength Analyte diffusivity Contact area Secondary reactions Time

Introduction Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) Membrane Assembly Device

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) Stir membrane (A) and the three different PTFE stir bar units, namely, (B) 622 mm 2 -surface PTFE stir bar, (C) 433 mm 2 -surface PTFE stir bar, and (D) 89 mm 2 -surface stir bar. (1) fluorene, (2) anthracene, (3) fluoranthene, (4) pyrene, and (5) benzo-anthracene.

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) 2016 Stir fabric phase extraction Fabric phase Device

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) 2016 Stir fabric phase extraction Fabric phase

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane liquid extraction (SMLE) (i) (ii) (iii) (v) (iv) (ii) (iii) Internal chamber Assembly Device

Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane liquid extraction (SMLE) Extraction modes 2 phases 3 phases An organic solvent wetting the membrane and inside the acceptor chamber An organic solvent wetting the membrane and an aqueous solution inside the acceptor chamber. A pH gradient is the driving force for the transference of the analytes

Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane liquid extraction (SMLE) Application to Bioanalysis Acidic sample HAA - + H + Alkaline acceptor phase NSAlDs

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2013 SMLE adapted to low sample volumes Applicable to moderate to large sample volume Not useful for lower volumes than 10 mL pH gradient SLM Acceptor phase Sample 2 mL Parafilm Adaptation Agitated, not stirred Several samples at a time

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2014 Stir membrane solid liquid liquid extraction Analyzing solid samples Sample Organic phase Acceptor solution Membrane Eppendorf SOLID-LIQUID extraction LIQUID-LIQUID extraction Methylparaben, ethylparaben, propylparaben, butylparaben and ethylparaben in lyophilized breast milk

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir C18 borosilicate disk Stir Nanohorns borosilicate disk C18 derivatization NHs immobilization

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Magnetically confined NPs Stir frit extraction MNPs Magnet Commercial polyethylene frit Iron wire SPE 3 mL SorbentFrit

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain