Chapter 7: Membrane Structure & Function
membrane a fluid mosaic of lipids, proteins, and carbohydrates. held together by hydrophobic interactions membrane lipids / proteins can drift laterally Membranes must be fluid to work properly.
Integral proteins are embedded in the membrane – Unilateral – – Transmembrane – Peripheral proteins attached to the membrane’s surface (cytoplasmic side )
Protein functions 1.Cell to cell recognition 2. enzymatic activity 3- signal transduction (hormones) 4- intercellular joining 5- attachment to ECM 6-Cell Transport:
Solubility characteristics: Nonpolar and hydrophobic molecules dissolve in the membrane and cross it with ease (e.g. hydrocarbons and oxygen) Polar (hydrophilic) molecules are dependent on size and charge Small molecules (e.g. H 2 O, CO 2 ) may be small enough to pass between membrane lipids Larger molecules (e.g. glucose) will not easily pass IONS- even small ones (e.g. Na +, H + ) have difficulty penetrating the hydrophobic layer.
Transport proteins integral transmembrane proteins that transport specific molecules or ions across biological membranes. Are highly specific
Passive Transport diffusion of a substance across a biological membrane does not require the cell to expend energy.
Osmosis – diffusion of water across a selectively permeable membrane
Hypertonic solution – a solution with a greater solute concentration Hypotonic solution – a solution with a lower solute concentration Isotonic solution – solutions with an equal solute concentration
Water potential – measure of the tendency for a solution to take up water by a selectively permeable membrane. Water potential of pure water is zero. -Solutes lower the water potential (ex -2) -Water flows from HIGH to LOW (water potential) -Water flows from hypotonic to hypertonic
Water Balance of Cells Without Walls In a hypertonic environment, an animal cell will shrivel. In a hypotonic environment, an animal cell will swell, and perhaps lyse (burst).
Organisms without cell walls prevent excessive loss or uptake of water by: Living in an isotonic environment Osmoregulating
Water Balance of Cells With Walls prokaryotes, some protists, fungi, and plants In a hypertonic environment, walled cells will plasmolyze (Plasmolysis)
a hypotonic environment Creates turgid cells = ideal for support Turgidity is the firmness or tension found in walled cells.
In an isotonic environment, the plant cells become flaccid (limp).
Facilitated diffusion – diffusion of solutes across a membrane with the help of transport proteins.
Active Transport ATP requiring process during which a transport protein pumps a molecule across a membrane against its concentration gradient.
Electrochemical gradient – diffusion gradient resulting from the combined effects of membrane potential and concentration gradient
Electrogenic pump – a transport protein that generates voltage across a membrane Na + /K + pump is the major electrogenic pump in animals
Exocytosis and Endocytosis Exocytosis – process of exporting macromolecules from a cell by fusion of vesicles with the plasma membrane Endocytosis – process of importing macromolecules into a cell by forming vesicles derived from the plasma membrane
three types of endocytosis: Phagocytosis – endocytosis of solid particles Pinocytosis – endocytosis of fluid droplets taken in as small vesicles.
Receptor-mediated endocytosis – importing specific macromolecules into the cells by the binding of specific ligands to receptors
Stages of Receptor-Mediated Endocytosis: Extracellular ligand binds to receptors in a coated pit Causes inward budding of the coated pit Forms a coated vesicle Ingested material is released from the vesicle Protein receptors are recycled to the plasma membrane