TFPI cofactor function of protein S: essential role of the protein S SHBG-like domain by Natalia Reglińska-Matveyev, Helena M. Andersson, Suely M. Rezende, Björn Dahlbäck, James T. B. Crawley, David A. Lane, and Josefin Ahnström Blood Volume 123(25): June 19, 2014 ©2014 by American Society of Hematology

Protein S enhancement of the TFPI-mediated inhibition of thrombin generation in protein S– depleted plasma. Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology

Enhancement of the TFPI-mediated inhibition of thrombin generation by WT protein S and protein S/Gas6 chimeras I to III. Thrombin generation was measured in protein S–depleted plasma supplemented with 50 μM phospholipids and 1 pM TF, in the presence or abse... Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology

Enhancement of TFPI in the inhibition of FXa by WT protein S and protein S/Gas6 chimeras I to III. FXa activity (0.5 nM) was followed in real time through cleavage of S-2765 (200 µM) at 405 mm in the presence of 25 μM phospholipids, the presence or absence... Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology

Enhancement of TFPI inhibition of FXa by protein S–, C4BP-, and C4BP-bound protein S. FXa activity (0.5 nM) was followed in real time through cleavage of S-2765 (200 µM) at 405 mm in the presence of 25 μM phospholipids, the presence or absence of TFPI (2 nM... Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology

Binding of WT protein S and protein S/Gas6 chimeras I to III to TFPI studied with SPR. A CM5 chip was coupled with TFPI immobilized to 2500 response units. Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology

Proposed mechanism of the protein S/TFPI/FXa complex assembly on a phospholipid surface. Natalia Reglińska-Matveyev et al. Blood 2014;123: ©2014 by American Society of Hematology