Innovative Analytical Solutions for the Dairy Industry

BactoCount IBC/IBCm Highly standardized method Rapid & accurate determination of Milk Total Flora & Somatic Cells. BactoCount IBC BactoCount IBCm

Overview BactoCount Method’s General Principle The BactoCount Universal Conversion Equation Methods Standardization: a prerequisite (ISO Standards) Findings from a 10 year- 13 country- Study BactoCount International Proficiency Ring Test Conclusions

BactoCount Method’s General Principle Flow Cytometry Total Flora (10’) 1.Degradation of milk biological interfering components by… chemical, heat and mechanical or ultrasonic treatment 2.Destruction of the bacterial aggregates to count Individual Bacteria (IBC) instead of Colony Forming Units (CFU) 3.Staining of the Bacteria DNA/RNA 4.Detection of Individual Bacteria (IBC) 5.IBC translated to Colony Forming Units (CFU) with a Conversion Equation (ISO 21187) Somatic Cells Mixing of Milk & Buffer Staining of the somatic cells DNA with fluorescent marker

BactoCount Method Standardization ISO 9001:2008 Production Certified Company Lyophilized Certified Bacteria/SCC Standard

The BactoCount Universal Conversion Equation The Conversion Equation includes all relevant variations Bacteria types and growth phase Storage conditions Regional influences & production conditions Seasonal variations Sample preservations For a robust Conversion Equation, you must use natural raw milk samples and evenly cover the entire working range of 100 samples/Log unit.

BactoCount Universal Equation (2010) BactoCount v. ISO Countries – 13 BactoCount – 8 Years – 4413 Samples Countries Brazil Czech republic Estonia France Germany Ireland Italy Lithuania Switzerland Turkey USA

BactoCount Universal Equation (2011) BactoCount v. ISO Countries – 15 BactoCount – 9 Years – 6504 Samples Countries Brazil Czech republic Estonia France Germany Ireland Italy Japan Lithuania Switzerland Turkey USA

BactoCount Universal Equation (2013) BactoCount v. ISO Countries – 22 BactoCount – 11 Years – 9735 Samples Countries Brazil Czech republic Estonia France Germany Ireland Italy Japan Lithuania Poland Switzerland Turkey USA Finland

BactoCount European Conversion Equation (2013) BactoCount v. ISO EU Countries – 22 BactoCount – 10 Years – 7706 Samples

BactoCount Universal Conversion Equation Robustness

BactoCount IBC ISO Accredited International Proficiency RT (CECALAIT) Protocol: 10 Preserved raw milk samples: kCFU 2 IBC standard RM (lyophilized) 1 Microsphere Working Solution RM (MS) Samples analyzed in duplicates Results reported in IBC

BactoCount IBC ISO Accredited International Proficiency Test 22 BactoCount – 14 Countries – 0% lab. Outside target Sr= 0,09 log SR= << 0,16 log Sy,x= 0,035 log << 0,40 log BactoCount fully complies with ISO requirements BactoCount Reproducibility is within the ISO Repeatability requirement

BactoCount IBC Accuracy (Universal CE) CECALAIT ISO International Proficiency Test BactoCount IBC v. ISO 4833

BactoCount IBC CECALAIT ISO International Proficiency Test

BactoCount Accuracy BactoCount v. ISO /IDF (Somatic Cells)

BactoCount IBC/IBCm Analytical Specifications Flow Cytometry Powerful Laser Very High Sensitivity Long Integration Time Outstanding Performances

BactoCount IBC/IBCm Real-time detection of potential contaminations in raw milk In the lab Rapid and highly accurate determination of: Bacteria Contamination in raw milk from cows, sheep, goats and buffalos. Bacteria contamination in individual animals, bulk tank, milk tankers etc. In the field Rapid control of the milk tankers To avoid silos contamination. Optimize milk segregation. Rapid control of the silos before processing Adjusts pasteurization process based on milk quality, detection of contamination etc.

BactoCount IBC/IBCm The BactoCount is the ultimate solution for rapid and accurate calculations of the bacteria and somatic cells in milk Real time, Highly Accurate tests (TF, SCC...) Simple to use (lab operators, truck drivers...) Dependable Versatile (open platform) Worldwide equivalence of analytical results (TF/SCC)

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