Page 1 Protein Crystallization and Structure Determination ---by Creative BiostructureCreative Biostructure

Page 2 Structure determination is usually a tedious and expensive process whereby the target macromolecule must be produced in relatively large quantities and purified in high concentrations. A successful experiment often requires expert knowledge of biochemistry, bioinformatics and molecular biology. Over the past decades, rapid technical advances in DNA cloning, protein expression and purification methodologies have made it generally easy to obtain enough amount of proteins for their structural study. Besides, numerous innovations and improvements have been made in solving protein structure using instrumental methods such as X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy and electron microscopy (EM). These efforts together have given rise to dramatic accumulation of 3-D structural data in the past few decades (Figure 1). The large number of protein structures will certainly yield valuable information to the rules for elucidating protein folding/unfolding mechanisms and understanding their biological functions.protein structure

Page 3 Creative Biostructure has a team of scientists who have rich experience in solving the structure of various protein targets, such as kinases, polymerases, chaperones and membrane proteins. Over the years, we have developed four unparalleled platforms, including X-ray crystallography, NMR, EM and membrane protein screening, to facilitate your research in structural biology.structural biology

Page 4 By using the most advanced platform apparatus and techniques, Creative Biostructure promises unbeatable precision in protein structure services with competitive prices. Protein crystallization: Creative Biostructure offers high-throughput UniCrys™ protein crystallization services with our state-of-the-art facilities. High-quality protein crystals can be obtained, which are suitable for X-ray diffraction studies. Structure determination: Creative Biostructure employs various strategies for the structure determination of macromolecules of your interest. Our scientists are experienced with phase determination of X-ray diffraction pattern, NMR spectroscopy and cryo-EM. We promise to provides the most efficient and economical strategies tailored to our customers’ requirement.

Page 5 Contact Creative Biostructure Mailing Address: 45-1 Ramsey Road Shirley, NY 11967, USA Address: Telephone Numbers: Website: and-structure-determination_13.htmhttp:// and-structure-determination_13.htm