Page 1 NMR Services —by Creative Biostructure

Page 2 NMR spectroscopy is a key analytical technique for structure elucidation of a wide range of materials from small molecules to compounds. The technique provides detailed molecular information that allows researchers have in- depth understanding of composition, chemical structure, morphology, and dynamics. NMR is particularly useful in analysis of pharmaceuticals, screening weak-binding compounds and developing into drug-like inhibitors for drug discovery. Supported by our NMR platform, Creative Biostructure offers high quality customized services ranging from production of labeled-proteins to acquisition and analysis of high-field NMR data for researchers in science and pharmaceutical industry.NMR

Page 3 Why Creative Biostructure? State-of-the-art instruments Senior scientists with extensive exerience Rapid turnaround time

Page 4 NMR services capabilities Compound structure determination Chemical composition analysis Molecular characterization Reaction kinetics examination Quality control Dynamics, disorder analysis Isotope-labeled protein production Quantitative analysis Drug screening and design NMR mapping for living cells

Page 5 Sample preparation guideline Labeling with isotopes: Protein NMR spectroscopy requires isotope enrichment, such as 15N, 13C, 31P, 19F in common. Samples with other isotope are also acceptable. Size: The molecular weight of biomacromolecule of a NMR sample is typically less than 50 KDa for high-resolution structure elucidation. Larger proteins or complexes can be studied to identify ligand/drug-binding site or to characterize the physical state. Amount: For solution NMR, a sample typically contains 1-10 mg/ml or 0.3 mM purified protein in a suitable buffer; for solid-state NMR, we require larger amount of samples, please talk to our specialist for more information. Stability: For most in-depth studies by NMR, the protein needs to be stable for several days at room temperature for data acquisition.

Page 6 Contact Creative Biostructure Mailing Address: 45-1 Ramsey Road Shirley, NY 11967, USA Address: Telephone Numbers: Website: services_28.htmhttp:// services_28.htm