Quiz#8 LC710 11/28/11 name___________ Q1:Given the following activity pattern for your enhancer/promoter complex: b-galactosidase activity 1000 A B C D E F LacZ 900 A B C D E LacZ A B LacZ 100 A E LacZ C D LacZ E F LacZ 200 B C D LacZ Make a conclusion about the enhancers as to how they work. Be Brief 4pts (1 sentence) Points will be taken off for being too descriptive. _______________________________________________________________________

Q2:Given the following scenario: Three Eukaryotic proteins where Estrogen binds to Protein A, thus causing Protein A to bind to Protein X, thus causing Protein X to bind to Protein B. Protein B is a DNA binding protein and typically found in the nucleus. Protein A and X can be found in the cytosol and nucleus. The DNA element Z has been identified as the binding site for Protein B. Describe (and draw) an in vivo assay that would confirm this scenario (4pts) E.C. (1pt) Why might you want to add Estrogen in your assay?_________________________________________________________________________ A X B