Vaccines

1 Edward Jenner 1796 Black pox (smallpox) Cow pox

Immunization 2 Passive immunization Active immunization Hepatitis A, B, tetanus, varicella, botulism Active immunization

3 Inactivated vaccines live virus propagation many viruses inactivation vaccine in cells heat, formalin purification

Attenuated (weakened) viruses 4 Live vaccines Attenuated (weakened) viruses live virus propagation propagation propagation attenuated virus vaccine

Recombinant vaccines adenovirus vector 5 pathogen adenovirus recombinant propagation recombinant vaccine pathogen virus gene of

Recombinant vaccines poxvirus vector rabies 6 vaccinia virus G protein gene rabiesvirus rabies G protein-expressing vaccinia virus [ vaccinia virus rabies virus

Avian (bird) influenza virus 7 Recombinant vaccines Avian (bird) influenza virus [

DNA vaccines 8 antigen gene pathogen recombinant plasmid plasmid mRNA protein free antigen genetic material recombinant plasmid muscle skin antigen complex on cell surface MHC-I gene gun

9 Subunit vaccines peptide vaccines antibody antigens

10 Toxoid vaccines Clostridium tetani

dying HIV–infected cell Anti-AIDS vaccine recombinant plasmid HIV Gag gene HIV INITIAL INJECTION recombinant plasmid 11 muscle adenovirus with Gag gene Gag protein BOOSTER SHOT, months later APC CELLULAR IMMUNE RESPONSE HUMORAL IMMUNE RESPONSE Gag protein fragment Inactive cytotoxic T cell helper T cell(CD4) activated cytotoxic T cell cytokines activated B cell antibodies APC: antigen presenting cell dying HIV–infected cell

Edible vaccines 3rd Generation GMOs 12 Agrobacterium plant cell recombinant plasmid Agrobacterium plant cell antibiotic resistance callus regeneration Vaccine potato

Vaccination with mosquitos 13 Vaccination with mosquitos Leishmania sp. Anti-Leishmaniasis vaccine in the mosquito saliva