Liquid Controller Client: Professor Ian Bird, Ph.D Advisor: Professor Paul Thompson, Ph.D Team Members: Joe Decker – Communicator Justin Gearing – BSAC Dan Miller – BWIG Steve Welch - Leader

Outline Background Problem Statement Current Design PDS Summary Design Components Design Alternatives Design Matrix Future Work Questions

Background Microscopy experiments Cells tested on a microscope tray Experiments test prevalence of calcium in the cell

Current Design Reagents added by hand Time kept manually Reagents switched manually

Problem Statement Current setup is: Design new, automated system Time Consuming Labor Intensive Error Prone Design new, automated system

Summary of PDS Design must be: Biologically friendly Automated Easy to use Reliable Transportable

Overall Design Electrically operated valves Controlled by program Power supply and wires stored in case Valves Case

Design: LabVIEW Program on/off times for each valve Saving formats allow quick plug-and-go Prints report of times, amounts of solution Interface (image): low voltage output, to be stored in case www.labjack.com

Design: Power Relay Boosts low-voltage output from LabVIEW interface to 12vdc needed for valves Requires wall outlet for 12 v source Also stored in case

Design: Valves and Panel Two-way normally closed isolation valves Teflon coating 12 VDC, 1.15 W Array of 8 to be mounted on panel www.nresearch.com

Inseparable Valve Panel Mounted directly to box Reconnect tubes to valves Pros: No chance for lost components Cons: Difficult to remount valves Opportunity for valves to break valves To Power Source To USB

Semi-Separable Valve Panel Panel can be disassembled from stand Attached to box for transport Pros: Cost Efficient User Friendly Components easily replaced Cons: Loose wires Panel Disconnect Power Box

Fully Separable Valve Panel Two separate structures Pros: Less chance of damage from transportation Cons: More expensive More parts to replace Wires must be reattached when transporting

Design Matrix Design User-Friendly (30) Cost (10) Total 30 20 10 15 85 Durability (30) Replaceable (15) Convenience (15) Cost (10) Total Inseparable 30 20 10 15 85 Semi-Separable 25 95 Fully Separable 5

Projected Budget $480.00: valves $40.00: Power Relay Board $130.00: LabVIEW interface $100.00: miscellaneous building supplies Total: $750.00

Future Work Finish programming Order parts Construction Testing

References www.labjack.com www.nresearch.com

Questions?