H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram showing the overlap of H. influenzae genes whose expression is up‐regulated or down‐regulated by the presence of corticosteroids in sBHI medium (in vitro) or in the mouse model of infection (in vivo). H. influenzae genes differentially regulated during growth in complex media and during lung infection in the presence of corticosteroids AVenn diagram showing the overlap of H. influenzae genes whose expression is up‐regulated or down‐regulated by the presence of corticosteroids in sBHI medium (in vitro) or in the mouse model of infection (in vivo). Genes that are divergently regulated in these conditions are not depicted in this Venn diagram but can be found in the complete RNA‐Seq dataset detailed in Supplementary Table S2.BCOG map detailing significantly enriched or scarce functional categories expressed in vitro and in vivo during the presence of corticosteroids.C, DComparison of relative fold changes between RNA‐seq and qRT–PCR results in vitro (C) and in vivo (D) in the presence of corticosteroids. All qRT–PCR results were normalized using the Cts obtained for the 16S rRNA amplifications run in the same plate. The relative levels of gene transcripts are determined from standard curves. Values given are the mean and standard deviation of triplicate measurements (three biological and three technical replicates). Chris S Earl et al. EMBO Mol Med. 2015;7:1018-1033 © as stated in the article, figure or figure legend