Turn in your Unit 2 Formative (rubric stapled) Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

Do Now – 11.22.16 Which two vectors carry plasmid DNA? Naked DNA and liposome Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus Which vector carries dsDNA? Adenovirus Which vector carries RNA? Retrovirus Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus Take out your 2.2.1 packet and Unit 2 Review Guide – turn to the vector table

2.2.1: Gene Therapy

Objective SWBAT choose the appropriate vector for each gene therapy scenario. SWBAT review for their Unit 2 Test.

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy Follow the worksheet – complete on looseleaf (determine the appropriate vectors for each condition – then explain which vector is the best) Answer and discuss the 2.2..1: conc questions.

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy

2.2.1: Gene Therapy Diseases caused by single gene mutations that cause defective proteins in somatic cells. The researchers test out the vector before actually trying it out on a patient or a patient’s cell. Cells are infected with the viral vector ex vivo (outside of the body) before an in vivo therapy is tried. The advantages of using viral vectors for gene therapy include: it can target specific cells, it can integrate into the host’s DNA. The disadvantages: can interrupt genes upon insertion, and it can cause an immune response.

2.2.1: Gene Therapy Gene therapy is the practice of inserting functional genes into a person’s genome to replace faulty genes. When choosing the appropriate vector you want to be sure the gene of interest will fit into the vector, the vector targets the right cells, and it should limit side effects. The keys to solving this problem are: 1) the type of cell that is the target of the vector [nerve cell] 2) the characteristics of the target cell [nerve cells do not divide] 3) the length of the gene to be carried by the vector [8,400 bp] Sounds like Herpes Simplex Virus would be an appropriate vector.

2.2.1: Gene Therapy Increases strength of athletes’ muscles. Could inject a gene that generates testosterone, growth hormone, or a muscle-strengthening protein. Opinion.

2.2.1: Gene Therapy Invasive - would need to isolate DNA, then sequence the DNA to see if the DNA sequence has changed compared to DNA found in the non targeted somatic cells. There have been many failures along with the successful cases. Many are still violently opposed to the experimentation. As of 2007, there have been 1,340 completed or approved gene therapy clinical trials in 28 different countries worldwide. The United States is responsible for 64.2% of all gene therapy trials. Other major contributors include the United Kingdom at 11.1%, Germany at 5.5%, and Switzerland at 3.1% (Edelstein et al. 2007).

Exit Slip Have you already started studying for your test? Why or why not?

Homework Do Now/Exit Slip Reflection Parent Signature EC Review Guide Unit 2 Test on Tuesday

Video https://www.youtube.com/watch?v=jAhjPd4uNFY