What is DNA Extraction? _ A routine procedure to collect DNA for subsequent molecular or forensic analysis. _ DNA is extracted from human cells for a variety.

Slides:

Advertisements

Similar presentations

Molecular Genetic.

Advertisements

DNA Extraction Outline Purpose of DNA extraction

Extracting DNA I. Purpose:

DNA Extraction from Cheek Cells

University of California Davis Partnership for Plant Genomics Education 2007 DNA Extraction from Strawberry.

Lab 6 Isolation Techniques

Plasmid DNA Isolation Exercise 8.

Extraction of Human DNA

General Genetics. 1. Be introduced to the laboratory techniques involved in DNA extraction. 2. Test DNA integrity using gel electrophoresis.

DNA Extraction Lab Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study.

بعض الأجهزه المستخدمة في الوراثة الجزيئية Explanation of some equipment and operation ways.

DNA Extraction Activity. Why extract DNA from a…….? Demonstrate that food actually has DNA in it! One study showed that some people think that traditionally.

DNA Extraction from Strawberries

Experiment Questions DNA Isolation.

A modern tool for genetics testing, forensic science, and research.

DNA ISOLATION. INTRODUCTION  DNA isolation is an extraction process of DNA from various sources. The scientist must be able to separate the DNA from.

Extraction and Quantitation of DNA From E. coli

How to get DNA from common food items.  A routine procedure to collect DNA for subsequent molecular or forensic analysis.DNAmolecularforensic  DNA is.

CAVEAT!!! Usually we do these procedures with a pure culture ◦ Grow bacteria in broth ◦ Streak on plate ◦ Grow single colony on broth.

Extraction of Human DNA

In order to Study DNA, it must be isolated (extracted) DNA is in all living tissues “If there are cells, there is DNA.” Isolating DNA DNA can be obtained.

Isolation and Purification of DNA from Escherichia coli GROUP 2 Chester Mancia Frances Miclat Mark Mosses Oliva HUB 42.

Genes in a Bottle DNA Extraction. Relevance of DNA isolation Isolation of DNA is often the first step before further analysis DNA profiling cloning disease.

Go Bananas! Introduction Tell you about DNA Show you how to extract DNA from a Banana.

What is DNA? DNA is a molecule that contains ALL of an organism’s genetic material and is therefore responsible for HEREDITY A strand of DNA contains many.

CAPE Biology Workshop on Concepts in Biotechnology & Genetic Engineering Prepared and presented by Dr. Marcia E. Roye.

Isolation of biological macromolecule Technology to simply go into a mixture and grab a single type of molecule is not readily available Instead use procedures.

TECHNIQUES USE IN GENETIC ENGINEERING

DNA extraction.

Extracting DNA from Eukaryotic Cells

Today: DNA Extraction! Shireen Rudina.

1. Be introduced to the laboratory techniques involved in DNA extraction. 2. Test DNA integrity using gel electrophoresis.

DNA Extraction from Strawberries Post Lab Protocol: Step by Step What was the purpose of Mashing the Berries? –physically break apart the tissues and.

DNA Extraction!. Why would we want to isolate an organisms DNA? List 3 reasons.

Lab. 6 DNA extraction from human blood. Be introduced to the laboratory techniques involved in DNA extraction. Test DNA integrity using gel electrophoresis.

DNA Long term storage of genetic information Double Helix Made up of nucleotides A, T, G, C Supercoiled to allow for efficient storage.

Rec. DNA Lab Wednesday, Jan 24, 2007 Isolation of Chromosomal DNA from Photobacterium leognathi.

Wed Review the structure & function of DNA

DNA Extraction Lab Pre – Lab Questions. Besides peas, what other items could possibly be used as a DNA source? What is the purpose of the blender in this.

McMush Debrief. UNKNOWNS Unknowns CarbsProteinsLipidsNucleic Acids A---- B-+-- C+--- D--+- E---+

Bell Work: (Do in your head, not on paper) Which of the following things has DNA? –Strawberry - a tree branch - A zebra –Grass - a hydra –Water - air While.

 DNA is found in the cell of every living thing.  Proteins that help DNA maintain it’s form may obscure our ability to see the DNA so they must be removed.

DNA extraction.

BTE 204: Fundamentals of Genetic Engineering

Extraction of Human DNA from blood

Our DNA Extraction Lab By Collena and Hallie. Step 1 We first had to decide if we wanted to extract DNA from a strawberry or our saliva. We first had.

Structure of the cell • Plasma membrane and membranes of organelles, nuclear envelope included • DNA located in nucleus • A lot of proteins around • Mitochondrial.

DNA Extraction from human blood

Extraction of Human DNA

Purpose of DNA Extraction

DNA Extraction and Isolation

DNA ISOLATION: Strawberry Lab

Extraction of DNA from Human Cheek Cells Activity

Done by :Sahar ALSubaie

Biology 201 Lab # 11 Fall 2017 Dr. Santos

Lab no. 6 DNA extraction from human blood

Pusat Kedokteran Tropis Fakultas Kedokteran - Universitas Mulawarman

DNA Extraction Lab Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study.

DNA EXTRACTION Protocol and notes 9/17/2018.

Activity 9 DNA Isolation.

DNA Extraction and Analysis: and 1.2.3

DNA Extraction Lab Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study. Bacterial cells.

محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)

DNA Extraction!.

Plasmid DNA Isolation.

DNA Extraction Lab Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study.

MOLECULAR BIOLOGY Lap2: DNA Extraction

DNA in my food? Information obtained from Mike Zeller, Biotechnology Outreach Education Coordinator.

Plasmid DNA Isolation.

Presentation transcript:

What is DNA Extraction? _ A routine procedure to collect DNA for subsequent molecular or forensic analysis. _ DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA you can test a newborn for a genetic disease, analyze forensic evidence, or study a gene involved in cancer. Steps to DNA Extraction 1. Breaking the cells open to expose DNA 2. Remove membrane lipids by adding detergent 3. Precipite DNA with an alcohol — usually ethanol or isopropanol. Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. This step also removes alcohol-soluble salt.