2016 Wagyu 101

WELCOME Introductions: Board Members Martha Patterson, AWA Office Manager Michelle Norton, Assistant Registrar Kelley Marcantel, Accounts Manager

TOPICS The DNA Submission/Registration Process Filling Out the New Combined Form Understanding Your DNA Results Transferring/Selling Your Cattle Website Navigation

DNA Submission/Registration Process Pull tail hair or collect blood FILL the card full of hair with follicles Drop blood to fill circle – let it dry! Label the card with animal ID Fill out submission form – include barcode from sample card on the form Mail 1 copy of form with DNA samples to GeneSeek Mail 1 copy of form with payment to AWA Office Please DO NOT mail samples to the AWA office

GeneSeek DNA Card This is the BARCODE ANIMAL ID HERE

Blood Card Hair Card

Filling Out Your Form General Guidelines: Legibility is critical, if the lab can’t read your information your results could be affected or delayed Accuracy is also critical, double check your forms for typos and mistakes, this again may affect or delay your results. Notes on/with the form to add clarity are encouraged. Let the lab and the office know if you have a special circumstance. Example: “these heifers lost their ear tags, need profile to compare…”

Animal ID

Contact Information Name - Ranch Name or Membership Name Member Name - Your name AWA Membership No. – Please always include this number. This can cause a delay in getting your DNA order processing. Email – Your results are sent via email so be sure to fill in this area. Animal Names – based on parents names, when parents change

Animal Information Area   Animal Information TO BE REGISTERED (Y or N) or Tattoo / Tag Animal ID Tag / Tattoo Loc: [L]eft , [R]ight or [B]oth Sex M F or S Animal Date of Birth mm/dd/yy Animal NAME Birth Weight (lbs) Calving Ease Score ( 1-5) Horn | Poll | Scurred Color: [B]lack [R]ed Service Code (Nat, AI or ET) Animal Registration Number (if known) Hair Card Sample Barcode Sample I.D. Number See Results Y Z 123 L M 01/01/13 KUROGE WASHU 65 1 H B ET FB12345 112713088005 5606720 To Be Registered – Y or N Animal ID or Tattoo/Tag - This is the animal’s permanent mark or tag which is unique to that animal. Location of Animal ID – Right Ear, Left Ear etc. Sample Barcode Number – This number is on the back of the DNA sample card. Sex (M/F/S) Animal Date of Birth (mm/dd/yy) Calf Name – Required for Registration only Birth Weight Calving Ease – 1 = unassisted Horned, Polled, Scurred Color – Color of coat Registration number if already registered DNA Card Barcode – Number is on the DNA card Sample ID Number – If already tested in a different order (assigned by lab) Emphasize Male-Female-Steer and also what true definition of Scurred is (a dehorned animal is not scurred)

Sire/Dam Area Information for Parents Sire 1** Registration Number   Sire 2** Registration Number Dam ** Registration Number FB1615 FB2501 FB12233 Registration Number - Please list the American Wagyu Association registration number If registering a F1, please indicate what breed the non-Wagyu parent is (typically Angus)

Test Area Clearly check the box below the test you want to have done on that animal. Wagyu Panels (option 1 & 2) – please note that these 2 Wagyu panels are a combination of popular tests put together and discounted. See the following page for details. When STR aka microsatellite markers are needed for overseas sales, mark the box below “STR markers for Export use”. DNA TEST REQUEST Wagyu Panel Wagyu Meat Panel Horned / Polled TEST Coat Color Test SNP Parentage BVD - PI (Hair or Tissue ONLY) JCP (F11, F13, CHS, B3) CL16 Test (ONLY) SCD FAT / TENDERNESS STR GENOTYPE (for Export Use) SNP GENOTYPE ONLY  

Submitting Additional Parents This is the area were you can list additional parents over the 3 included – additional charges apply Offspring ID - From this order – ear tag / tattoo Sample Barcode – From this order AWA registration number of Parent

Mailing Instructions Send Samples and a copy of the forms to GeneSeek – Please do not send samples to the AWA office, this will only cause a delay in getting your samples processing. Send a Payment and a copy of the forms to American Wagyu Association. Please remember to send a copy of the forms to the AWA office so that we can confirm with GeneSeek to be sure they have received and recorded your order correctly.

Understanding Your DNA Results Sample ID number – Where do you find this Sire/Dam individual qualifications or exclusions Trio qualifications – Why all three have to qualify together. Types of results: “Not on File” “Finished “Failed”” “NR” and “Cancelled” SCD values – AA, VA, VV what they mean Tenderness values – 1-10 what is the average for Wagyu V=Valine and A=Alanine are the amino acids. Alanine has a lower melting point than Valanine.

American Wagyu Breed Categories Eligibility of animals FULLBLOOD WAGYU 100% Fullblood sire and dam Animals registered in the AWA or those registered in Japanese Wagyu Herdbook

PUREBRED WAGYU 93.75% Wagyu blood of it’s registered Wagyu sires and dams No white above the flank or in front of navel

PERCENTAGE WAGYU Females must have at least 50% Wagyu Blood The percentage of Wagyu blood is the “average” of Wagyu blood of both sire + dam as on AWA Registration Certificate

Where to find Wagyu Blood Percentage: WAGYU HERDBOOK

Transferring/Selling Your Cattle

Transferring/Selling Your Cattle Buyers Information – Please be as thorough as possible. We use this area to create the title for the new owner. Date of Sale – critical for future offspring registrations. Seller’s Signature - required

TRANSFERS Continued…………Don’t forget CALF-AT-SIDE INFORMATION Animal ID

Website Navigation http://www.wagyu.org

Questions?????