M-SeriesTM Compact MRI Systems

Aspect’s M-Series Systems Compact Simple to use Safe Quiet Maintenance-free Affordable

Advantages of Aspect Imaging MRI Systems User friendly & sample preparation is routine without the cost, complexity, and infrastructure needs of conventional MRI Useful in vivo scans can be acquired in 10 minutes or less Ex vivo scans (scans of multiple fixed specimens) can be automated to run overnight

System Components Compact magnet with integrated electronics Application-specific RF coils and animal handling beds Total small animal solution Anaesthesia Heating Physiological monitoring ECG and/or respiration triggering Auto load/eject system for HT ex- vivo imaging

Longitudinal Growth of a Brain Tumor Collaborators Rinat Abramovitch, Hadassah Hebrew University Medical Center, Israel Yael Schiffenabuer, Aspect Imaging, Israel Catherine Brami, Aspect Imaging, Israel Abraham Nyska, Tel Aviv University & Consultant in Toxicologic Pathology, Israel

Biological Model and Objective Model: Gl-261 glioma cells stereotactically injected into the right brain hemisphere of CB6F1 mice Objective: Longitudinal evaluation of tumor growth

Longitudinal Evaluation of Tumor Growth In Vivo MRI (T2) Day 15 Day 17 Day 20 axial coronal Exponential tumor growth Acquisition parameters: FSE : TR/TE 2500/74ms , slice thickness 1mm, 256x256, FOV 40mm, Nex 8, scan time 13.2min Time (days) resolution 156 mm; slice thickness 1 mm; acquisition time 13 min

Tumor Segmentation Day 17 – coronal Tumor volume 6.6 mm3 Injection site Injection site Acquisition parameters: FSE : TR/TE 3440/80ms , slice thickness 0.7mm, 256x256, FOV 40mm, Nex 40, scan time 57min Tumor volume 6.6 mm3

Brain Tumor (In vivo MRI)

Variability in Tumor Size (n=4, day 15) In Vivo MRI (T2) mouse 1 mouse 4 mouse 3 mouse 2 resolution 156 mm slice thickness 1 mm acquisition time 13 min Acquisition parameters: FSE : TR/TE 2500/74ms , slice thickness 1mm, 256x256, FOV 40mm, Nex 8, scan time 13.2min

Ex Vivo MRI vs Histology Day 15 Day 20

Summary & Comment In-vivo and ex-vivo MRI evaluation provided a way to follow the time-related growth of an induced tumor in the brain and to determine the volume of the tumor This model demonstrates the utility of using MRI for longitudinal studies and would be useful for testing the efficacy of anti-cancer drugs

Hepatocarcinoma growth in vivo Collaborators Julia Vilensky, Itay Spector, Yossi Lavie, & Nati Ezov, Harlan Biotech Israel, Nes Ziona, Israel Yael Schiffenabuer, Aspect Imaging, Israel Catherine Brami, Aspect Imaging, Israel Abraham Nyska, Tel Aviv University & Consultant in Toxicologic Pathology, Israel

Biological Model and Objective Model: Orthotopic hepatocellular carcinoma induced in BALB/c nude mice in two stages Subcutaneous injection of HepG2 cell suspension in the flank of donor mice Intrahepatic implantation of tumor fragments obtained from donors into liver lobe of BALB/c nude mice Objective: Evaluation of orthotopic tumor growth

Tumor Growth in vivo 2 weeks 3 weeks Slice thickness = 1 mm 4 weeks Acquisition parameters: FSE: TR=2651 ms; TE=80ms; FOV=64 mm; ST=1mm; Matrix 256x252, Acq time = 10.34 min Acquisition time = 10.3 minutes

Tumor Volume is Determined From Multiple Slices – In Vivo MRI 4 weeks Acquisition parameters: FSE: TR=2651 ms; TE=80ms; FOV=64 mm; ST=1mm; Matrix 256x252, Acq time = 10.34 min

Quantification of Tumor Volume in vivo 3 weeks 4 weeks 5 weeks Acquisition parameters: FSE: TR=2651 ms; TE=80ms; FOV=64 mm; ST=1mm; Matrix 256x252, Acq time = 10.34 min ROI Color Voxels Volume mm³ tumor 3 weeks Red 1619 25.2969 tumor 4 weeks 5649 88.2656 tumor 5 weeks red 10899 170.297 Volume (mm3) Time post implantation (weeks)

Summary & Comment In Vivo MRI evaluation enabled to follow the time- related growth of this orthotopic tumor in the liver, and to delineate the volume of the tumor over time. This model demonstrates the potential utility of using MRI for testing the efficacy of anti-cancer drugs.