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From: Kinase-Dependent Differentiation of a Retinal Ganglion Cell Precursor Invest. Ophthalmol. Vis. Sci.. 2006;47(1):427-438. doi:10.1167/iovs.05-0340 Figure Legend: RGC-5 cells increased the expression of RGC markers after differentiation. (A) Untreated (left) or SS-differentiated (right) RGC-5 cells incubated for 24 hours stained with mouse anti-glutamate receptor (anti-NMDAR1; clone 54.1) followed by goat anti-mouse IgG. (B) Untreated (left) or SS-differentiated (right) RGC-5 cells incubated for 24 hours with rabbit anti-Thy-1 polyclonal IgG stain followed by goat anti-rabbit IgG. (C) Protein levels of Thy-1 and MAP-2 measured by immunoblot analysis was increased after SS differentiation. Actin was used as the loading control. (D) Changes in Thy-1 and MAP-2 expression were quantified by densitometry. Thy-1 and MAP-2 protein levels were increased after 3 days of differentiation with 316 nM SS. RGC-5 actin content was unchanged. Date of download: 9/26/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.