Go to this link and complete questions 1 +2 only!

Slides:

Advertisements

Similar presentations

Serological tests (Antigen antibody interactions)

Advertisements

Research Techniques Made Simple: Enzyme Immunoassay and ELISA

Enzyme Linked Immunosorbent Assay

ELISA Enzyme Linked Immunosorbent Assay. Definitions  Antibodies (also known as immunoglobulins abbreviated Ig) are gamma globulin proteins that are.

Instructions for using this template. Remember this is Jeopardy, so where I have written “ Answer ” this is the prompt the students will see, and where.

Monoclonal Antibodies NIS Uralsk. Medical use of Antibodies Monoclonal antibodies (mAbs) are pure, single antibody types that are industrially produced.

Antigens & Antibodies: reactions, detection, and applications.

Enzyme-Linked Immunosorbent Assay ELISA 1Dr. Nikhat Siddiq.

(Enzyme Linked Immunosorbent Assay)

Application of immunological tests

Antigen antibody reactions

Lab 24 Goals and Objectives: EDVOKIT#271: Simulation of HIV-1 Detection: ELISA Work in pairs: ten groups possible Perform as indicated (supplemental packet.

ELISA Equipment. INTRODUCTION TO ELISA ELISA, or Enzyme-Linked Immunosorbent Assay, are immunological procedures used to determine the presence of antibodies.

A CALL TO ARMS. KOCH’S POSTULATES In the 1880s German doctor, Robert Koch, developed a series of methods for identifying which organism was the cause.

HIV & AIDS What is it?.

Enzyme-Linked Immunosorbent Assay [ELISA] BCH 462[practical] Lab#5.

ELISA Assay. What Is It? Enzyme immunoassay (EIA) is a test used to detect and quantify specific antigen-eliciting molecules involved in biological processes,

Ex. 27: HIV ELISA, AIDS Diagnostic Tool. Human Immuno- deficiency Virus (HIV) First diagnosed in 1981 Over 20 million deaths worldwide, over a half million.

ELISA Testing. What is ELISA Technology? Enzyme-Linked Immunosorbant Assay.

ELISA Enzyme-Linked Immunosorbent Assay. What is an assay? An analysis done on a substance to determine the presence of a target substance and sometimes,

A SEMINAR ON IMMUNO-DIAGNOSTIC TECHNIQUES

Immunological Tests A harmful agent can be detected

Enzyme Linked Immuno Sorbent Assay (ELISA) Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique.

Human Immunodeficiency Virus (HIV) First diagnosed in 1981 Over 20 million deaths worldwide, over a half million in the United States Over 40 million currently.

Biochemical instrumental analysis - 12 Dr. Maha Al-Sedik 2015 CLS 332.

Lab 24 Goals and Objectives: EDVOKIT#271: Simulation of HIV-1 Detection: ELISA Work in pairs: ten groups possible Perform as indicated (supplemental packet.

Introduction to Viruses. Viruses are ‘disease causing agents’. Nucleic Acid surrounded by a protein coat called a capsid Some have a additional external.

ELISA Enzyme-linked Immunosorbant Assay Detects the presence of minutes quantities of either an antibody or an antigen Important diagnostic tool in many.

HIV and ELISA Structure of the human immunodeficiency virus (HIV) and its replication in helper T cells. How HIV causes the symptoms of AIDS. Why antibiotics.

HIV. Learning Objectives Outline the structure of HIV Discuss the means of transmission and how the spread can be controlled.

Enzyme Linked Immunosorbent Assay

© 2004 Wadsworth – Thomson Learning Chapter 19 Diagnostic Immunology.

HIV Human immunodeficiency Enveloped, icosahedral, single stranded linear, RNA It belongs to the Retrovirus family that is.

MONOCLONAL ANTIBODIES What are they? How are they produced? What potential uses?

Enzyme-linked immunosorbent assay (ELISA)

EDVOKIT#271: Simulation of HIV-1 Detection: ELISA

Detection of Infectious diseases Using Antibodies

Lab # 6 ELISA Enzyme – Linked Immuno Sorbent Assay.

Enzyme Linked Immunosorbent Assay

Immunoassays are tests that take advantage of the specificity of antibodies to their protein epitotes. Qualitative example: pregnancy test kits, HIV.

Absorbance: Enzyme Linked Immunosorbent Assays (ELISA)

Enzyme-Linked Immunosorbent Assay ELISA

Antibody-Antigen Reactions

ELISA Immuno ExlorerTM HIV/AIDS Diagnostic Tool

Immunofluorescence Indirect Direct proof of antibodies (Ab)

ELISA Immuno ExlorerTM HIV/AIDS Diagnostic Tool

Globular Protein Made of amino acid chains

Enzyme-Linked Immunosorbent Assay [ELISA]

AIDS Simutest MCB 151 FA17.

Specific Immunity and Immunization

Defense against infectious diseases

Acquired Immunodeficiency Syndrome

A CALL TO ARMS.

Specific (Adaptive) Immunity

ELISA Immuno ExlorerTM HIV/AIDS Diagnostic Tool

Vaccines and Opportunistic Infections

ELISA Immuno ExlorerTM HIV/AIDS Diagnostic Tool

Immune System Practice Test

ELISA Immuno ExlorerTM HIV/AIDS Diagnostic Tool

Immune System Practice Test

Research Techniques Made Simple: Enzyme Immunoassay and ELISA

Tracking the Outbreak of HIV using ELISA Assays

Immune System Practice Test

Enzyme Linked Immuno sorbent Assay

Experimental Systems and Methods

Bio-Rad Curriculum and Training Specialist

Presentation transcript:

Go to this link and complete questions 1 +2 only! SPECIFICATION POINTS: Structure of the human immunodeficienc y virus (HIV) and its replication in helper T cells. How HIV causes the symptoms of AIDS. Why antibiotics are ineffective against viruses. The use of antibodies in the ELISA test. http://PYBUIOJ.exampro.net Go to this link and complete questions 1 +2 only!

ELISA – detecting HIV Enzyme Linked Immuno-sorbant Assay/ SPECIFICATION POINTS: Structure of the human immunodeficienc y virus (HIV) and its replication in helper T cells. How HIV causes the symptoms of AIDS. Why antibiotics are ineffective against viruses. The use of antibodies in the ELISA test. ELISA – detecting HIV Enzyme Linked Immuno-sorbant Assay/ Can help you detect the presence of antigens and how many antigens. https://www.youtube.com/watch?v=RRbuz3VQ100

Direct ELISA A test to detect the presence of HIV antigens 4. Finally substrate is added and only will be broken down by the enzyme on bound antibodies. If there is a colour change, this is a positive result for HIV An antibody that is complimentary to the HIV/pathogen antigen is attached to the glass dish. 3. A secondary antibody is added, this one is complimentary to the HIV antigen too but on an alternative site. These either become fixed if HIV antigen present or washed away if not. This secondary antibody is bound to an enzyme that can cause a change in colour if substrate is added. 2. The sample from a person (usually blood or urine) is added to the dish. If the person has the HIV antigen (hence has been infected), then they will attach to the antibody and become fixed. Any unbound antigens/fluids are washed away.

Indirect ELISA A test to detect the presence of HIV antibodies The HIV/pathogen antigen is attached to the glass dish. The sample from a person (usually blood or urine) is added to the dish. If the person has HIV antibodies (hence has been infected), then they will attach to the antigen and become fixed. Any unbound antibodies/fluids are washed away. A secondary antibody is added, this one is complimentary to the HIV antibody that could be present in the body fluid. These either become fixed if HIV antibody present or washed away if not. This secondary antibody is bound to an enzyme that can cause a change in colour if substrate is added. Finally substrate is added and only will be broken down by the enzyme on bound antibodies. If there is a colour change, this is a positive result for HIV

SPECIFICATION POINTS: Structure of the human immunodeficienc y virus (HIV) and its replication in helper T cells. How HIV causes the symptoms of AIDS. Why antibiotics are ineffective against viruses. The use of antibodies in the ELISA test.

SPECIFICATION POINTS: Structure of the human immunodeficienc y virus (HIV) and its replication in helper T cells. How HIV causes the symptoms of AIDS. Why antibiotics are ineffective against viruses. The use of antibodies in the ELISA test. How could an ELISA test also become quantitative (i.e. indicate the amount of antigen in the blood)