MIDDLE SCHOOL GENETICS Tt t t MIDDLE SCHOOL GENETICS SINGLE TRAIT PUNNETT SQUARE ¼

TOOLS TO KNOW PARENT’S GENES A PUNNET SQUARE IS A TOOL USED TO PREDICT THE POSSIBLE GENOTYPES FOR THE OFFSPRING OF TWO KNOWN PARENTS. PARENT’S GENES

T,t TT, tt Tt, Gg TERMS TO KNOW ALLELES HOMOZYGOUS HETEROZYGOUS DIFFERENT FORMS OF A TRAIT THAT A GENE MAY HAVE T,t HOMOZYGOUS AN ORGANISM WITH TWO ALLELES THAT ARE THE SAME TT, tt HETEROZYGOUS AN ORGANISM WITH TWO DIFFERENT ALLELES FOR A TRAIT Tt, Gg

Tt, Gg T OR G t or g TERMS TO KNOW HYBRID DOMINANT RECESSIVE SAME AS HETEROZYGOUS Tt, Gg DOMINANT A TRAIT THAT DOMINATES OR COVERS UP THE OTHER FORM OF THE TRAIT REPRESENTED BY AN UPPERCASE LETTER T OR G RECESSIVE THE TRAIT BEING DOMINATED OR COVERED UP BY THE DOMINATE TRAIT REPRESENTED BY A LOWER CASE LETTER t or g

TERMS TO KNOW PHENOTYPE TALL, SHORT, GREEN, WRINKLED GENOTYPE THE PHYSICAL APPEARANCE OF AN ORGANISM (WHAT IT LOOKS LIKE) TALL, SHORT, GREEN, WRINKLED GENOTYPE THE GENE ORDER OF AN ORGANISM (WHAT ITS GENES LOOK LIKE) TT, GG, Tt, gg Gg, tt RATIO THE RELATIONSHIP IN NUMBERS BETWEEN TWO OR MORE THINGS 3:1, 2:2, 1:2:1

HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE THE PARENTS’ ALLELES GO ON THE OUTSIDE OF THE SQUARE B B BB X bb b b

HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE THE PARENTS’ ALLELES GO ON THE OUTSIDE OF THE SQUARE DROP THE LETTERS ON THE TOP, INTO EACH SQUARE B B b B b B b MOVE EACH LETTER ON THE SIDE, INTO EACH SQUARE b THE ORDER DOES NOT MATTER IN THE BOXES, BUT UPPERCASE FIRST IS A GOOD RULE B b B b

HOW TO USE A MONOHYBRID (ONE TRAIT) PUNNETT SQUARE WHAT DO THE RESULTS SHOW? RESULTS: B B PHENOTYPIC: 100% BLACK 4:0 RATIO, BLACK TO BROWN IF B IS THE DOMINANT ALLELE FOR BLACK b Bb Bb AND b IS THE RECESSIVE ALLELE FOR BROWN GENOTYPIC: 100% Bb 4:0 ALL Bb b Bb Bb THEN WE CAN MAKE PREDICTIONS ABOUT THE OUTCOMES

HOW TO USE A PUNNETT SQUARE WHAT ARE THE RESULTS? LET’S LOOK AT ANOTHER PUNNETT SQUARE AND PREDICT THE OUTCOME T t PHENOTYPIC: 75% TALL 25% SHORT 3 TO 1 RATIO: TALL TO SHORT T T T T t T IS THE DOMINANT ALLELE FOR TALLNESS GENOTYPIC: 1TT: 2Tt: 1tt 1:2:1 RATIO 25 %TT, 50% Tt, 25% tt t T t t t t IS THE RECESSIVE ALLELE FOR SHORTNESS

PRACTICAL APPLICATION OF PUNNETT SQUARES THE ALLELES OF A PARTICULAR SPECIES OF DOG CAN BE EITHER D (NORMAL HEIGHTH) OR d (DWARF). THE HETEROZYGOUS (Dd) AND HOMOZYGOUS DOMINANT (DD) FORM OF THIS DOG LOOK THE SAME (TALL). IF YOU FOUND A STRAY DOG OF THIS BREED, HOW COULD YOU DETERMINE ITS GENOTYPE?

PRACTICAL APPLICATION OF PUNNETT SQUARES COULD A DOG BE CROSSED WITH ANOTHER DOG TO DETERMINE IF HE WAS PUREBRED FOR TALLNESS? WHAT GENOTYPE SHOULD THE DOG HAVE THAT IS BEING USED FOR THE CROSS?

PRACTICAL APPLICATION OF PUNNETT SQUARES D D IF THE DOG IS PUREBRED (DD), IT DOESN’T MATTER WHAT YOU CROSS IT WITH, THE OFFSPRING WILL ALWAYS LOOK LIKE THE DOMINANT. DD DD D D DD DD D D D D D DD DD d Dd Dd d d Dd Dd Dd Dd

PRACTICAL APPLICATION OF PUNNETT SQUARES WHAT WOULD BE THE MOST EFFECTIVE CROSS FOR DETERMINING IF THE DOG IS HETEROZYGOUS (Dd) ? CROSSING IT WITH A PUREBRED (DD) WILL NOT HELP. WHAT WOULD THE RESULTS BE IF YOU CROSSED IT WITH ANOTHER HETEROZYGOUS? WHAT WOULD THE RESULTS BE IF YOU CROSSED IT WITH A HOMOZYGOUS RECESSIVE (dd)? D d D d D DD Dd d Dd dd d d Dd dd Dd dd

PRACTICAL APPLICATION OF PUNNETT SQUARES D d D d D DD Dd d Dd dd d Dd dd d Dd dd THE HETEROZYGOUS CROSS WOULD ONLY GIVE YOU A 25% CHANCE OF THE RECESSIVE TRAIT APPEARING. THE MOST EFFECTIVE CROSS WAS USING THE HOMOZYGOUS RECESSIVE. THIS WOULD GIVE A 50% CHANCE OF THE RECESSIVE TRAIT APPEARING. THIS PROCESS IS CALLED A TEST CROSS. IN A LITTER OF DOGS, IF A RECESSIVE DOG APPEARS, THEN YOU KNOW THAT THE ORIGINAL DOG WAS NOT A PUREBRED.

TAKS FORMATTED ITEMS ANSWER: 50% IN DROSOPHILA MELANOGASTER (FRUIT FLIES), RED EYE COLOR (R) IS DOMINANT OVER BROWN EYE COLOR (r). IF THE FLIES IN THE PICTURE WERE CROSSED, WHAT PERCENT OF THEIR OFFSPRING WOULD BE EXPECTED TO HAVE BROWN EYES? ANSWER: 50%

TAKS FORMATTED ITEMS H h H 4 1 h 3 2 WHICH OF THE FOLLOWING HAS THE hh GENOTYPE? 1 & 3 2 4 NONE H 4 1 h 3 2 2. WHICH OF THE FOLLOWING IS A TRUE STATEMENT? INDIVIDUAL 4 IS RECESSIVE INDIVIDUALS 1 & 3 ARE HETEROZYGOUS INDIVIDUAL 2 IS DOMINANT ALL INDIVIDUALS ARE FEMALE

TAKS FORMATTED ITEMS B b B BB Bb b Bb bb 3. IF B IS THE ALLELE FOR BLACK FUR AND b IS THE ALLELE FOR WHITE FUR, WHAT PERCENT WOULD BE BLACK? 25% 50% 100% 75% B BB Bb b Bb bb 4. WHAT FRACTION IS HOMOZYGOUS DOMINANT IN THE ABOVE CROSS? 1/2 1/4 1/3 3/4

TAKS FORMATTED ITEMS B B B BB BB b Bb Bb 5. IN THIS CROSS, WHAT IS THE RATIO OF BB TO Bb? 3 : 1 4 : 1 2 : 2 0 : 4 B BB BB b Bb Bb

BIBLIOGRAPHY Mendel picture provided by wikipedia.com Dog and tree pictures provided by Microsoft ClipArt Graphics from TEA/ TAKS information booklet Middle School Science Grade 8 Dice graphic from Microsoft Clipart