RASSF2DSARAH-GF (b) MST1-F (a) M F G 70 kD a 55 kD b 36 kD

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RASSF2DSARAH-GF (b) MST1-F (a) M F G 70 kD a 55 kD b 36 kD Additional file 3. Binding analysis of RASSF2DSARAH and MST1 in co-precipitation. Plasmids, that express the indicated GST-Flag-tagged-constructs (-GF) and Flag-tagged-constructs (-F) were transfected into HEK293 cells. Deletion of SARAH domain of RASSF2 was performed according to Cooper et al. 2009. After one day, total protein was extracted and Flag-tagged proteins were precipitated with anti-Flag-agarose (F) or glutathione sepharose (G). Samples were separated on a 10% PAGE gel and blotted. The precipitated and co-precipitated proteins were determined with anti-Flag-antibodies. The sizes of a protein marker (M) are indicated.