= Supporting information of Citrate-modified silver nanoparticles as a colorimetric probe for simultaneous detection of four triptan-family drugs Sweta K. Laliwala,† Vaibhavkumar N. Mehta,† Jigneshkumar V. Rohit,† and Suresh Kumar Kailasa* aApplied Chemistry Department, S. V. National Institute of Technology, Surat-395 007, India *Corresponding author, Phone: +91-261-2201730; Fax: +91-261-2227334 E-mail: sureshkumarchem@gmail.com, skk@ashd.svnit.ac.in †These authors contributed equally to this work. AgNO3 NaBH4 Trisodium citrate Ag Ag AgNPs = Triptan Drugs Scheme S1. Schematic illustration of the colorimetric detection strategy for triptan drugs based on citrate-capped Ag NPs.

Table S1: The structures, molecular weights and solubility behavior of four-triptan family drugs g/mol Solubility pKa values Rizatriptan 269.4 Water 9.56 Naratriptan 333.46 9.70 Sumatriptan 413.49 9.54 Zolmitriptan 287.35 Methanol 9.55

(a) (b) (c) (d) Figure S1. Effect of time on the UV-visible absorption spectra of citrate-capped Ag NPs after addition of (a) rizatriptan (0.0 min to 2.5 min) (b) naratriptan (0.0 min to 2.5 min) (c) sumatriptan (0.0 min to 3.0 min) and (d) zolmitriptan (0.0 min to 3.5 min).

pH Blank 2 3 4 5 6 7 8 9 10 (a) pH Blank 2 3 4 5 6 7 8 9 10 (b) Figure S2. Effect of pH on the generation of UV-visible absorption spectra of induced aggregation of citrate- capped Ag NPs with (a) zolmitriptan (1 mM) and (b) naratriptan (1 mM).

pH Blank 2 3 4 5 6 7 9 10 8 Figure S3. Effect of pH on the SPR peak of citrate-capped Ag NPs without addition of analytes.

(a) aromatic -N-H stretching C-H stretching aliphatic -N-H stretching -C=C- stretching -C-N stretching (b) Figure S4. FT-IR spectra of (a) rizatriptan and (b) citrate-capped Ag NPs with rizatriptan

(a) -C-N stretching -C=C- stretching aliphatic -N-H stretching aromatic -N-H stretching C-H stretching -S=O stretching (b) Figure S5. FT-IR spectra of (a) naratriptan and (b) citrate-capped Ag NPs with naratriptan

(a) aromatic -N-H stretching -C=C- stretching aliphatic -N-H stretching -C-N stretching C-H stretching -S=O stretching (b) Figure S6. FT-IR spectra of (a) sumatriptan and (b) citrate-capped Ag NPs with sumatriptan

(a) -C=C- stretching -C-N stretching aromatic -N-H stretching aliphatic -N-H stretching -C=O stretching C-H stretching (b) Figure S7. FT-IR spectra of (a) zolmitriptan and (b) citrate-capped Ag NPs with zolmitriptan

i ii iii iv v vi vii Figure S8. UV-visible absorption spectra for interference study of citrate-capped Ag NPs (i) citrate-capped Ag NPs + different drugs (amoxicillin, ampicilline, cortisone, cyclosporine, methylcobalamine, tramadol) (ii) citrate-capped Ag NPs + triptan drugs (iii) citrate-capped Ag NPs + different drugs + triptan drugs (iv) citrate-capped Ag NPs + different drugs + naratriptan (v) citrate-capped Ag NPs + different drugs + rizatriptan (vi) citrate-capped Ag NPs + different drugs + sumatriptan and (vii) citrate-capped Ag NPs + different drugs + zolmitriptan

i ii iii iv v vi vii Figure S9. UV-visible absorption spectra for interference study of citrate-capped Ag NPs (i) citrate-capped Ag NPs (ii) citrate-capped Ag NPs + different drugs (Rabeprazole, Indapamide, Folic acid, Carbidopa, Linsinopril, Afluzosin) (iii) citrate-capped Ag NPs + different drugs + triptan drugs (iv) citrate-capped Ag NPs + different drugs + naratriptan (v) citrate-capped Ag NPs + different drugs + sumatriptan (vi) citrate-capped Ag NPs + different drugs + rizatriptan and (vii) citrate-capped Ag NPs + different drugs + zolmitriptan

Figure S10. UV-visible absorption spectra of citrate-capped Ag NPS in the presence of different triptan drugs (naratriptan, sumatriptan, rizatriptan and zolmitriptan)

20 µg/ml 2 µg/ml Figure S11. UV-visible absorption spectra for different concentrations of rizatriptan in rizatriptan benzoate tablet. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of rizatriptan (20 µg/mL to 2 µg/mL) 20 µg/ml 2 µg/ml Figure S12. UV-visible absorption spectra for different concentrations of sumatriptan in sumatriptan and naproxen tablet. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of sumatriptan (20 µg/mL to 2 µg/mL)

20 µg/ml 2 µg/ml Figure S13. UV-visible absorption spectra for different concentrations of zolmitriptan in Zolmist nasal spray. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of zolmitriptan (20 µg/mL to 2 µg/mL)