Chapter 17a How Genes Work

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Presentation transcript:

Chapter 17a How Genes Work From Gene to Protein Chapter 17a How Genes Work

Objectives Understand the relationship between genes and proteins Understand the overall process by which genetic information is converted to polypeptides Recognize where and why transcription occurs Recognize where and why translation occurs Understand relevant vocabulary

One Gene, One Enzyme Work of George Beadle and Edward Tatum Bread mold nutrition Inability to metabolize a particular AA is the result of an inability to produce necessary enzymes Genes dictate the production of a specific enzyme Later modified to reflect the condition that all proteins are not enzymes nor are all made of a single polypeptide: One Gene-One Polypeptide

Nucleotide Bases Are The Source Of Information For The Building Of Proteins

3 sequential nucleotides code for 1 AA 4 nucleotide bases cannot independently code for 20 different AA Pairs of bases would only account for 16 AA Triplet bases would give us 64 possible AA that could be coded, exceeding the required amount necessary

Linking Genetic Information to the Synthesis of Proteins If Genes (bits of information on DNA) contain knowledge of how to assemble a polypeptide, then there must be a process by which information on the DNA is conveyed to the protein making machinery of the cell

Overview Transcription: the synthesis of RNA under the direction of DNA players include: DNA messenger RNA RNA Polymerase Occurs in the nucleus Translation: Synthesis of a polypeptide under the direction of mRNA mRNA ribosomal RNA transfer RNA Occurs in the cytosol

Messenger RNA (mRNA) Conveys Information Via Codons Only one strand of the double stranded DNA molecule is involved in transcription (Template Strand) Complimentary nucleotide triplets of mRNA are called a Codon (remember that U is substituted for T as a complimentary pair for A)

Codons Deciphered Marshall Nirenberg (1961) deciphered the meaning of the codon UUU All 64 possible codon “words” have been determined Redundancy occurs Start and Stop codons occur Reading frame: sequence and groupings of the “words”.