On-site, laboratory-grade, rapid-response testing Definitive results. Decisive actions. On-site, laboratory-grade, rapid-response testing

The Problem Laboratory Testing OndaVia System Traditional lab-based water analysis is slow Laboratory Testing OndaVia System Collect sample (1-L bottle) Ship to lab (at 4°C) Wait for lab to test sample Wait for results Receive (paper) test report Collect sample (<10-mL) Test (on-site, <5 minutes) Analytics Three-week process for >>$50 On-site, 5-minute test for $50

Broad Applicability Pre-treatment or pre-concentration methods OndaVia’s technology replaces lab testing for any compound in any liquid. Chemicals Applications Amines Corrosion control Triazines H2S scavenging, pesticides Se, As, Cr Waste water TCE, DBP Environmental, drinking water Lead Drinking water @ low-ppb levels Pre-treatment or pre-concentration methods Instrument and software Analysis kit

Technology and Product Based on our eSERS™ detection backed by eleven issued US Patents Monoethanolamine repeatability Average: 27.8±3.9 ppm Average: 76.4±5.1 ppm

Selenium analysis Regulatory limits (drinking water) Applications 50-ppb, although some States are lower Applications Refinery waste water Coal-fired power plants / flue-gas desulfurization water Current methods ICP/MS and colorimetry

Selenium speciation Test is specific for Se(VI) Facility ICP/MS (ppb) OndaVia (ppb) 1 (untreated) 400 405 2 (untreated) 370 420 1 (treated) 55 ND 2 (treated) 160 1 (treated, ox) -- 70 2 (treated, ox) 145 Test is specific for Se(VI) Se reduced during biological treatment of waste water Oxidize treated water using bleach and/or H2O2 at high pH to convert all Se(IV) to Se(VI) Fieldable speciation test: First measure Se(VI) Oxidize to determine total Se(IV) is the difference

Arsenic analysis Arsenic exists in natural waters predominantly as arsenite [As(III)] and arsenate [As(V)] Regulatory limit: 10-ppb total As in drinking water But As(III) is more hazardous that As(V) Current methods: Total via colorimetry – arsenic is converted to As(III) and then to arsine gas Speciation via HG-AAS

Arsenic speciation Arsenite [As(III)] Arsenate [As(V)]

Target Specifications Arsenic and selenium Trichloroethylene (TCE) Five minute test <10-ppb detection limit Ability to speciate As(III) vs As(V) Se(IV) vs Se(VI) Selenium commercially available, arsenic available for beta testing Five minute test 5-ppb detection limit Still early stage (Phase I) TCE

On-site, laboratory-grade, DIY, rapid-response testing First ever commercial, quantitative analytical method using surface-enhanced Raman spectroscopy Broad applicability from anions to metals to VOCs Applications from QA/QC to process control to environmental monitoring and remediation Contact Mark Peterman OndaVia, Inc. 510-576-0476 info@ondavia.com Arsenic and TCE work funded under NIH/NIEHS SBIR Phase I grants 1R43ES025083 and 1R43ES026513