Prof. Joel C. Glover, PhD Laboratory for Neural Development and Optical Recording Dept of Molecular Medicine, University of Oslo Director, Norwegian Center for Stem Cell Research Oslo University Hospital, Oslo, Norway joel.glover@medisin.uio.no www.stemcellnorway.org Expertise in: Brain and spinal cord development, spinal cord injury (avian and mammalian models) Electrophysiology High throughput optical recording of neural network activity (calcium imaging, voltage sensitive dye imaging) Motor systems (locomotion, descending control, vestibular system and balance) Human ES cell and iPS cell-based in vitro differentiation and disease models (head of the Norwegian National Core Facility for Human Pluripotent Stem Cells), particular focus on motor system diseases (ALS, spinocerebellar ataxia, dystonia) but also schizophrenia, autism, bipolar disorder 3D cell printing (EnvisionTech 3D-Bioprinter) Neuronal tracing (invented method of tracing with conjugated dextrans), fluorescence and confocal microscopy (multiple systems) Live cell imaging using fluorescence (2-photon confocal) and bioluminescence (Ivis Spectrum µCT) Magnetic micro- and nanoparticle-based in vivo tracking of stem cells in animal models (MRI) Gene and protein expression profiling (immunohistochemistry, in situ hybridization, qPCR, RNAseq, phosphoflow cytometry) https://www.ncbi.nlm.nih.gov/pubmed/28033684 https://www.ncbi.nlm.nih.gov/pubmed/26730404 https://www.ncbi.nlm.nih.gov/pubmed/26019332 https://www.ncbi.nlm.nih.gov/pubmed/23990976 https://www.ncbi.nlm.nih.gov/pubmed/23140086 https://www.ncbi.nlm.nih.gov/pubmed/22490338

Expected benefits and activities during participation in BIONECA: Collaborate with groups using human stem cells for neurological disease modeling and potential treatment (gene editing, 3D printing, improved in vivo tracking protocols, recording and modeling of neuronal network activity, modulation of synaptogenesis) Collaborate on generating improved neuronal differentiation protocols and combined neuron-glia culture systems Coolaborate with groups in developing and implementing new imaging modalities Collaborate with groups interested in developing 3D cell printing for replicating human neural tissue using human pluripotent stem cells (combination of differentiation protocols, functionalized biomaterials, dynamic imaging platforms) Would like to join other partners to develop new project applications Foreseen maximum contribution: in WG1, WG2, WG4 Also interested in WG3 (am a developmental neuroscientist with great interest in how neural tissue and neural networks arise during embryonic development)