LAB SERVICES FOR POISON CENTRE 24 hrs Lab Service for Basic Lab Investigations. THREE CATEGORIES Basic Lab Investigation RFT, LFT, CBC, Urine R/E etc.. Specialized Investigation ABG Electrolytes by ISE CPK, LDH, Amylase, Blood Alcohol, Serum Cholinestrese Toxicological Analysis TLC – Thin layer – Chromatography OP, OC & Caramate – For Drugs. HPLC & Spectorophometer

BASIC LAB INVESTIGATION Semi Auto Analysesr – 1.5 to 2.0 Lakhs Cell Counter or Manual Method – 4 -5 Lakhs SPECIALIZED INVESTIGATIONS ABG 4 – 5 Lakhs Sampling – Clot Maintenance of instrument Calibration Quality Control EQAS Internal Quality Control Electrolytes by ISE Highly Sensitive and accurate CPK, LDH, Amylase commercial kits are available

BLOOD ALCOHOL Principle ADH Ethanol + NAD + ----- Acetaldehyde+NADH+H+ Alcohol dehydrogenase (ADH) catalyzes the oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD to NADH2. The change in absorbance at 340nm is directly proportional to the alcohol concentration in the sample.

SPECIMEN COLLECTION AND STORAGE The site of venipuncture should be disinfected only with aqueous disinfectants such as zephiran or merthiolate. Alcohol or other volatile disinfectants must not be used. Whole blood may be collected using citrate, heparin or oxalate as the anticoagulant.3 However, fluoride used at 5mg/dl of blood is the best preservative. Samples may be stored at 2-80C for several days and may not show appreciable loss of alcohol, when well stoppered.4 Serum samples may be assayed without deproteinization. Serum Cholinestrese

TOXICOLOGICAL ANALYSIS Collection of Samples Urine A urine specimen should be obtained, before the administration of any drugs. Usually a 50ml sample without added preservatives is sufficient for a comprehensive series of tests. Stomach Contents It is very important to collect and send the first sample of washing, atleast 50ml to carry out a wide variety of tests, without added preservatives. Blood Samples which should be taken on admission of the patient are 10ml of heparinised blood

Solvent Layer Fraction F Sample Extract With Ether Aqueous Layer (pH8) Ether Acid Fraction A Basic Fraction D Weak Acid Fraction B Ether Fraction E Neutral Fraction C Solvent Layer Fraction F

THIN LAYER CHROMATOGRAPHY Chromatography is the technique for the separation of mixtures by continuous distribution between two phases, one of which is mobile while the other is stationery. Silicagel Coated on Aluminium Sheets – Stationery Phase Solvent – Mobile Phase

THIN LAYER CHROMATOGRAPHY REQUIREMENTS ORGANOPHOSPHATES CARBAMATES SOLVENT Hexane Acetone Dichloroethane Diethylether SPRAY Bromium vapour Congored P- nitroaniline Sodiumnitrite Sodium hydroxide CONTROL Monocrotophos Triazophos Malathion Parathion Sevin Bagon Carbofuran

REQUIREMENTS OF TLC TLC glass chamber TLC aluminium plates Capillary tubes Appropriate solvent, spray TLC sprayer Ultraviolet lamp Approximate cost for each test is Rs 150

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