Thin-layer chromatography analysis of [3H]-labeled lipid fraction of the small intestine. Thin-layer chromatography analysis of [3H]-labeled lipid fraction.

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Thin-layer chromatography analysis of [3H]-labeled lipid fraction of the small intestine. Thin-layer chromatography analysis of [3H]-labeled lipid fraction of the small intestine. Mice were equipped with a duodenal cannula, but no lymph cannula, and were intraduodenally infused with a lipid emulsion containing labeled TG (the label was on the FA moieties of the TG) for 6 h. At the end of the study, the small intestines were divided into 4 equal segments, M1 (A), M2 (B), M3 (C), and M4 (D). The extracted lipid fraction was separated by thin-layer chromatography into TG, diacylglycerol (DAG), monoacylglycerol (MONO), and fatty acids (FA). *P < 0.05, statistically significant difference between WT and KO as determined by t-test (n = 6–8). Values are means ± SE. Alison B. Kohan et al. Am J Physiol Gastrointest Liver Physiol 2013;304:G1128-G1135 ©2013 by American Physiological Society