The Effects of the Perchlorate Ion in Simulated Martian Soil on Solanum lycopersicum Seed Germination in Microgravity Sabrina Benitez (Co-Principal Investigator)

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The Effects of the Perchlorate Ion in Simulated Martian Soil on Solanum lycopersicum Seed Germination in Microgravity Sabrina Benitez (Co-Principal Investigator) Juan Pablo Flanagan (Co-Principal Investigator) Sofia Escobar (Co-Principal Investigator) Laura Nikstad (Teacher Facilitator) International Baccalaureate at Lamar Academy McAllen, Texas

Mars: Recent discoveries of water on Mars led us toward the study of seed germination and sustainability on Mars The Martian (2015 film): The film’s accurate depiction of life and plant growth on Mars inspired our experimental design and sparked our interest Mauna Loa (Volcano): This volcano in Hawaii is the source of our Martian soil simulant

How does the perchlorate ion in simulated Martian soil affect Solanum lycopersicum seed germination in microgravity?

Experimental Design

Experimental Design Group 1 Group 2 Group 3 Earth Conditions Earth gravity Earth soil with 15 tomato seeds Distilled water Fixative Martian Soil Earth gravity Martian simulated soil (with sodium perchlorate) with 15 tomato seeds Distilled water Fixative Martian Soil & Microgravity Microgravity Martian simulated soil (with sodium perchlorate) with 15 tomato seeds Distilled water Fixative 3.0 mL distilled water 10.6 g soil 0.03 g sodium perchlorate NaClO4

Analyzing Results Dry weight of the germinated seedlings Count germinated and ungerminated seeds Dry and weigh Radicle Length Measure radicle length Count lateral roots Examine lateral root position

Hypothesis How does the perchlorate ion in simulated Martian soil affect Solanum lycopersicum seed germination in microgravity? Limited seed growth in Group 2 and Group 3 High solute (perchlorate) concentration in soil Could inhibit osmosis Radicle Length and Lateral Root Structure Perchlorate → shorter radicals Microgravity → lateral root growth evenly distributed along radical rather than concentrated at the end

Ground Truth Challenges Seed count Initial seed count too low Trials conducted to optimize seed count Water volume Too little → no growth Too much → drown the seeds FME Mini-Lab Size Limited volume Shape challenges Extract the seedlings without destroying them Disperse water throughout the soil sample Soil types Initial soil = chunky and dry Sifted to form powdery, fine grain soil Allows better diffusion of water Easier to access germinated seeds without destroying them

Image References The Hubble Heritage Team. Mars Near Opposition 1995-2005: 2003. Digital image. HubbleSite. NASA, 3 Nov. 2005. Web. 9 June 2016. <http://hubblesite.org/newscenter/archive/releases/2005/34/image/j/>. A Still from The Martian. Digital image. Matt Damon's The Martian Rules North American Box Office. NDTV Movies, 26 Oct. 2015. Web. 9 June 2016. <http://movies.ndtv.com/hollywood/matt-damons-the-martian-rules-north-american-box-office-1236546>. Mauna Loa. Digital image. Wired. N.p., n.d. Web. 9 June 2016. <https://www.wired.com/wp-content/uploads/2016/01/GettyImages-491821494.jpg>. The Martian: potato growth on Mars. Digital image. Modern Farmer. N.p., n.d. Web. 9 June 2016. <Modernfarmer.com>. Dicotyledonous Root. Digital image. Open Stax CNX. RICE, n.d. Web. 9 June 2016. <https://cnx.org/contents/pPKTz_LG@1/211---Anatomy-of-dicotyledenou>. Germination Plant Picture. Digital image. Free Photos. N.p., n.d. Web. 9 June 2016. <http://all-free-download.com/free-photos/germination_plant_picture_169076.html>. A new plant emerging from the seed during germination. Digital image.Study. N.p., n.d. Web. 9 June 2016. <http://study.com/academy/lesson/what-is-seed-germination-definition-process-steps-factors.html>.