Specimen Collection Guide for Cattle or Horses with Suspected Rabies, West Nile Virus Encephalitis or Bovine Spongiform Encephalopathy (BSE) Adapted from.

Slides:

Advertisements

Similar presentations

Instructions for users This slide presentation provides an overview of the diagnostic and laboratory testing aspects of JE. Below many of the slides, there.

Advertisements

Case of the month August 2006 Cavalier King Charles Spaniel, m, 3.5 y.

Smallpox Vaccination Laboratory Support Department of Health and Human Services Centers for Disease Control and Prevention December 2002.

Any of the following risk factors within 3 weeks (21 days) before onset of symptoms 1,2 : Contact with blood or other body fluids of a patient known to.

Mosquito-borne Arbovirus Surveillance in West Virginia Rachel Radcliffe, DVM, MPH CDC Career Epidemiology Field Officer Division of Infectious Disease.

West Nile Fever and Encephalitis From Mayoclinic.com.

WNV Human Case Investigation and Reporting Kimberly Signs, DVM Michigan Department of Community Health.

Algorithms and Testing Results for West Nile/Arbovirus Testing (2002) used at Michigan Department of Community Health H. Kapoor, P. Clark, F. P. Downes,

DIAGNOSIS OF WEST NILE VIRUS INFECTION IN ANIMALS Jon S. Patterson, DVM, PhD, DACVP Roger K. Maes, DVM, PhD Diagnostic Center for Population and Animal.

Testing algorithms used at Bureau of Labs Michigan Department of Community Health Information on testing algorithms for processing and reporting serological.

West Nile Virus Timeline  1937  First Case reported  1950  Virus studied in Egypt  1957  Outbreak in Israel  1960  Equine cases  1999  Appeared.

Causative Agent Virus Infects Humans, Birds, Mosquitoes, Horses and Other Mammals.

1 Module 5 Supplemental Information Laboratory Diagnostics, Specimen Collection, and Biosafety Issues.

Ebola Facts November 3, /3/14 Source: Centers for Disease Control and Prevention. This guidance is current as of Nov. 3, 2014 from

Post-Mortem Examinations Veterinary Science Extension Veterinary Medicine Texas AgriLife Extension Service College of Veterinary Medicine and Biomedical.

DEPARTMENT OF HEALTH RESEARCH INSTITUTE FOR TROPICAL MEDICINE Ebola Virus Disease Diagnosis Catalino S. Demetria, DVM.

Laboratory Investigation

MURRAY VALLEY ENCEPHALITIS ALERT in NE Victoria..(per DPI bulletins March 2011) Introduction Murray Valley encephalitis (MVE) virus is a type of arbovirus.

BY CINDY RAMEY West Nile Virus. West Nile virus (WNV) is a mosquito-borne zoonotic arbovirus Family: Flaviviridae Genus: Flavivirus Japanese Encephalitis.

West Nile Virus Jo Hofmann, MD State Epidemiologist for Communicable Disease Washington State Department of Health Focus on clinical aspects of human infection.

Instructions for users This slide presentation provides an overview of the components of a population-based surveillance system for JE. Please use this.

Hema Kapoor MD. SM (NRM) Virology Section manager Bureau of laboratories Michigan Department of community Health Lansing Michigan Laboratory Issues and.

Surveillance, Epidemiology, and Tracing Surveillance Part 1: The Surveillance Plan Adapted from the FAD PReP/NAHEMS Guidelines: Surveillance, Epidemiology,

Laboratory Issues and West Nile Virus Hema Kapoor MD. SM (NRM)

Theresa L. Smith, MD, MPH Division of Vector-Borne Infectious Diseases Centers for Disease Control and Prevention Fort Collins, CO West Nile Virus Epidemiology.

Serological Tests Dr. Mahmood Yaseen Hachim M.B.Ch.B, M.Sc. Medical Microbiology 2010 RAK Medical and Health Sciences University RAK College of Denatal.

Update on Assay Development George J. Dawson, Ph.D. Infectious Diseases: Core R & D Abbott Laboratories West Nile Virus.

1 An Introduction to the Viruses. 2 Viral Components All viruses have capsids- protein coats that enclose & protect their nucleic acid Viruses may have.

DISEASE, RECOVERY AND DIAGNOSIS PETER H. RUSSELL, BVSc, PhD, FRCPath, MRCVS Department of Pathology and Infectious Diseases, The Royal Veterinary College,

Arboviral Surveillance in Maryland, 2003 Kimberly C. Mitchell, MPH West Nile Virus Coordinator January 29, 2004.

Lab Diagnosis of Viruses Dr Syed Suhail Ahmed College of Medicine Qassim University.

RABIES Rabies belongs to Rhabdovirus It is the only human Rhabdovirus It is bullet-shaped, enveloped, helical, single stranded,

LAB. DIAGNOSIS OF VIRUSES 5 methods are used for diagnosis in the virology laboratory: 1.Direct microscopy 2.Cultivation of viruses 3.Serology 4. Detection.

West Nile Virus Kimberly Signs, DVM Michigan Department of Community Health.

TSE Advisory Committee October 25, 2001 Center for Food Safety and Applied Nutrition Food and Drug Administration Washington, DC Topic 3.

Avidity determination of IgG in diagnosis of tick-born encephalitis Hana Zelená Jiří Januška Jan Raszka Virology department, National Reference Laboratory.

Polio. Poliomyelitis, often called polio or infantile paralysis, is an acute viral infectious disease which is spread from person-to-person via the.

Surveillance, Epidemiology, and Tracing Surveillance Part 2: Implementing Surveillance Adapted from the FAD PReP/NAHEMS Guidelines: Surveillance, Epidemiology,

Test 1Test 2Test 3Test 4Test 5Test 6 Sensitivity Specificity PPV NPV Performance.

Case # 4 Clinical history An adult horse Acute onset of CNS signs.

Recent Serologic Studies with Potential Impact on WNV Blood Screening Wayne Hogrefe, Harry Prince Focus Technologies.

Dengue fever caused by dengue virus (DENV), a member of Flaviviridae leads to large global disease burden. Detection of immunoglobulin M (IgM) and nucleic.

Quick Insights on Some Viral Issues Dr. Haya Al-Tawalah Clinical Virologist.

- Is a clear, colorless body fluid.. - bathes the..

RABIES Rabies belongs to Rhabdovirus It is the only human Rhabdovirus It is bullet-shaped, enveloped, helical, single stranded,

Animal Health Status in Jordan All Animal Diseases are obligatory notifiable and controlled under MOA law and regulation. All Animal Diseases are obligatory.

Introduction to Neuroanatomy and Terminology. Main Regions of the Nervous System Two Main Divisions –Central Nervous System –Peripheral Nervous System.

DENGUE CASE INVESTIGATION REPORT CDC Dengue Branch and Puerto Rico Department of Health 1324 Calle Cañada, San Juan, P. R Tel. (787) ,

Diagnostic Testing for Zika Virus Frederick S. Nolte, PhD, D(ABMM), F(AAM) Professor and Vice-Chair for Laboratory Medicine Department of Pathology and.

Microbiology Unit 7 Chapter 39 Sample Collection and Handling Copyright © 2015 by Mosby, an imprint of Elsevier Inc. All rights reserved.

Unexplained Neurologic Illness in Children – Malkangiri, Odisha, India, 2014 Authors: Priyakanta Nayak1, Mohan Papanna1, Aakash Shrivastava1, Pradeep.

VIRAL INFLUENZA.

Infection Control Q and A APIC Greater NY Chapter 13 May 17, 2017 Beth Nivin BA MPH NYC DOHMH Communicable Disease Program

Samples collection.

Family Rhabdoviridae. Rabies virus.

Ethicon Endo-Surgery, Inc.

Copyright © 2017 American Academy of Pediatrics.

From: Clinical and Laboratory Diagnosis of Dengue Virus Infection

RESULTS AND DISCUSSION

LAB. DIAGNOSIS OF VIRUSES

Chapter 22: Diseases of the nervous system

Rebekah Doyle, Ph.D student

Diagnosing Infections

Fatal human rabies infection confirmed with skin biopsy specimen

Anatomy of the Nervous System

Figure 1 Diagram illustrating the sites for lung tissue specimen collection using minimally invasive tissue sampling ... Figure 1 Diagram illustrating.

Is an inflammation of cerebral tissue typically accompanied by meningeal inflammation, caused by an infection or other source.

Management Of Lassa Fever in a Resource Limited Setting: Experience From Irrua Specialist Teaching Hospital Main author: Gloria Esoimeme Co-authors:

Presentation transcript:

Specimen Collection Guide for Cattle or Horses with Suspected Rabies, West Nile Virus Encephalitis or Bovine Spongiform Encephalopathy (BSE) Adapted from guidelines of the Centers of Disease Control (CDC), Washington Animal Disease Diagnostic Laboratory and Texas Veterinary Medical Diagnostic Laboratory

Introduction Rabies, West Nile encephalitis, and Bovine Spongioform encephalopathy (BSE) are uncommon but important causes of neurological illness in horses and cattle. Appropriate specimen collection is essential for laboratory testing. *Do not split the brain sagittally as cross sections are needed. These slides were developed to assist veterinarians with specimen collection and submission. WSU’s Washington Animal Disease Diagnostic Laboratory (WADDL) in Pullman performs WNV and BSE testing; the Department of Health (DOH) does rabies testing. If you are not sure what specimens to submit, please call WADDL at (509) 335-9696. If you have rabies testing questions, please call DOH (206) 418-5500.

Review of Dorsal Brain Caudal brainstem Rostral brainstem is under cerebellum

Rabies Rabies diagnosis requires fresh (NOT FIXED) brain tissue sampled from the brainstem and cerebellum A full cross-section of the mid-cerebellum and subjacent rostral brain stem are needed for a valid test because rabies virus antigen can localized unilaterally The entire cerebellum and underlying brainstem is the optimal sample Keep the specimen cold (refrigerated) Call the local public health department. They will help arrange for shipping and rabies testing The Department of Health Public Health Laboratory (Shoreline) does rabies testing

Rabies Sampling Site

West Nile Virus Encephalitis The most common clinical signs include ataxia, weakness of hindlimbs, recumbency, muscle fasciculation and death. Many affected horses are afebrile. Antibody testing (serology) is appropriate when possible. Post mortem: Virus and lesions in horses are only found in nervous tissue: usually in the brain stem and thoracolumbar spinal cord.

West Nile Virus Diagnosis Serology IgM antibody to WNV demonstrated by antibody capture ELISA during acute disease. Sample: Cerebrospinal Fluid (CSF) and/or blood serum (red top tube) Polymerase Chain Reaction (PCR) Detection of viral RNA in CNS tissue (postmortem) Sample: Formalin-fixed or fresh /frozen whole brain (including caudal brainstem) +/- spinal cord (thoracolumbar is optimal) Submit to WSU / WADDL (Pullman) See diagram for taking brain samples for rabies and WNV

WNV and Rabies Sampling Specimen between lines for rabies testing; remainder of brain for WNV testing Brainstem

Bovine Spongiform Encephalopathy BSE: The specimen required for BSE testing is the “V” shaped obex on the dorsal aspect of the caudal brainstem Take the sample from caudal to the obex (caudally) to the cerebellar peduncle (rostrally) (see attached photograph) BSE sampling site is caudal to rabies sampling site BSE and rabies testing require fresh brain (NOT FORMALIN FIXED) Submit BSE tests to WSU / WADDL (Pullman). Contact USDA-APHIS before submitting BSE sample.

BSE Specimen Collection Obex “V” Shaped Obex

BSE and Rabies Specimen Collection Specimen between black and white lines for rabies testing. Specimen caudal to (behind) white line for BSE testing Brainstem