Title: Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis Jounal: Plant Cell.

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Title: Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis Jounal: Plant Cell Report Authours: Hiroyasu Kitashiba1, Kumiko Taguchi1, Ikuyo Kaneko1, Kiyofumi Inaba1, Shuji Yokoi2, 3, Yoshihito Takahata2, and Takeshi Nishio1. Affiliations and addresses of the authors: 1. Graduate School of Agricultural Science, Tohoku University 2. Faculty of Agriculture, Iwate University 3. Present address Graduate School of Life and Environmental Science, Osaka prefecture University Corresponding author: Hiroyasu Kitashiba Graduate school of Agricultural Science, Tohoku University 1-1, Tsutsumidori-Amamiyamachi, Sendai, Miyagi, 981-8555, Japan e-mail: hkitashiba@m.tohoku.ac.jp Tel: +81-22-717-8651 Fax: +81-22-717-8654

a Bra029631 (Bnm4D-84; 2,765 bp) HM-DH1: CTA (Leu274) CRS1 : GTA (Val274) zf-Ring finger domain (320-360) 5’ 2765bp 3’ SNP1. HM-DH1: AGT (Ser298) CRS1: AAT (Asn298) SNP2. HM-DH1: GAG (Glu300) CRS1: GAC (Asp300) SNP3. HM-DH1: CTT (Leu301) CRS1: CAT (His301) 100bp b Bra016537 (CLE-like; 288 bp) Signal Peptide 5’ 3’ SNP1. HM-DH1: GTT (Val19) CRS1: TTT (Phe19) SNP2. HM-DH1: GTA (Val20) CRS1: GCA (Ala20) 50bp c Bra016644 (HSP70B; 1,953 bp) Three conserved regions Peptide binding domain 5’ 3’ 100bp HM-DH1: AGG (Arg251) CRS1: AAG (Lys251) Supplementary Fig. 1. Differences of amino-acids by non-synonymous substitution for each gene between ‘HM-DH1’ allele and ‘CRS1’ allele.